Impact of natural oil and higher hydrocarbons on microbial diversity, distribution, and activity in Gulf of Mexico cold-seep sediments.

Orcutt, Beth N., Joye, Samantha B., Kleindienst, Sara, Knittel, Katrin, Ramette, Alban, Reitz, Anja, Samarkin, Vladimir, Treude, Tina and Boetius, Antje (2010) Impact of natural oil and higher hydrocarbons on microbial diversity, distribution, and activity in Gulf of Mexico cold-seep sediments. Deep-Sea Research Part II-Topical Studies in Oceanography, 57 (21-23). pp. 2008-2021. DOI 10.1016/j.dsr2.2010.05.014.

[img] Text
Orcutt_etal_2010_DSR.pdf - Published Version
Restricted to Registered users only

Download (1181Kb) | Contact

Supplementary data:

Abstract

Gulf of Mexico cold seeps characterized by variable compositions and magnitudes of hydrocarbon
seepage were sampled in order to investigate the effects of natural oils, methane, and non-methane
hydrocarbons on microbial activity, diversity, and distribution in seafloor sediments. Though some
sediments were characterized by relatively high quantities of oil, which may be toxic to some
microorganisms, high rates of sulfate reduction (SR, 27.9714.7 mmol m2 d1), anaerobic oxidation of
methane (AOM, 16.276.7 mmol m2 d1), and acetate oxidation (2.7470.76 mmol m2 d1) were
observed in radiotracer measurements. In many instances, the SR rate was higher than the AOM rate,
indicating that non-methane hydrocarbons fueled SR. Analysis of 16S rRNA gene clone libraries
revealed phylogenetically diverse communities that were dominated by phylotypes of sulfate-reducing
bacteria (SRB) and anaerobic methanotrophs of the ANME-1 and ANME-2 varieties. Another group of
archaea form a Gulf of Mexico-specific clade (GOM ARC2) that may be important in brine-influenced,
oil-impacted sediments from deeper water. Additionally, species grouping within the uncultivated
Deltaproteobacteria clades SEEP-SRB3 and -SRB4, as well as relatives of Desulfobacterium anilini, were
observed in relatively higher abundance in the oil-impacted sediments, suggesting that these groups of
SRB may be involved in or influenced by degradation of higher hydrocarbons or petroleum byproducts.

Document Type: Article
Keywords: Geochemistry; Methane, Sulfate reduction, Methanogenesis, Petroleum, Biodegradation, Ecology; R.V. Sonne
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-MG Marine Geosystems
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-JRG-A2 Seafloor Warming
AWI
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.dsr2.2010.05.014
ISSN: 0967-0645
Expeditions/Models/Experiments:
Date Deposited: 23 Nov 2010 11:02
Last Modified: 28 Jun 2019 12:02
URI: http://oceanrep.geomar.de/id/eprint/10095

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...