Distribution Analysis of Hydrogenases in Surface Waters of Marine and Freshwater Environments.

Barz, Martin, Beimgraben, Christian, Staller, Torsten, Germer, Frauke, Opitz, Friederike, Marquardt, Claudia, Schwarz, Christoph, Gutekunst, Kirstin, Vanselow, Klaus Heinrich, Schmitz, Ruth, LaRoche, Julie, Schulz, Rüdiger, Appel, Jens and Rodriguez-Valera, Francisco (2010) Distribution Analysis of Hydrogenases in Surface Waters of Marine and Freshwater Environments. Open Access PLoS ONE, 5 (11). e13846. DOI 10.1371/journal.pone.0013846.

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Abstract

Background

Surface waters of aquatic environments have been shown to both evolve and consume hydrogen and the ocean is estimated to be the principal natural source. In some marine habitats, H2 evolution and uptake are clearly due to biological activity, while contributions of abiotic sources must be considered in others. Until now the only known biological process involved in H2 metabolism in marine environments is nitrogen fixation.
Principal Findings
We analyzed marine and freshwater environments for the presence and distribution of genes of all known hydrogenases, the enzymes involved in biological hydrogen turnover. The total genomes and the available marine metagenome datasets were searched for hydrogenase sequences. Furthermore, we isolated DNA from samples from the North Atlantic, Mediterranean Sea, North Sea, Baltic Sea, and two fresh water lakes and amplified and sequenced part of the gene encoding the bidirectional NAD(P)-linked hydrogenase. In 21% of all marine heterotrophic bacterial genomes from surface waters, one or several hydrogenase genes were found, with the membrane-bound H2 uptake hydrogenase being the most widespread. A clear bias of hydrogenases to environments with terrestrial influence was found. This is exemplified by the cyanobacterial bidirectional NAD(P)-linked hydrogenase that was found in freshwater and coastal areas but not in the open ocean.
Significance
This study shows that hydrogenases are surprisingly abundant in marine environments. Due to its ecological distribution the primary function of the bidirectional NAD(P)-linked hydrogenase seems to be fermentative hydrogen evolution. Moreover, our data suggests that marine surface waters could be an interesting source of oxygen-resistant uptake hydrogenases. The respective genes occur in coastal as well as open ocean habitats and we presume that they are used as additional energy scavenging devices in otherwise nutrient limited environments. The membrane-bound H2-evolving hydrogenases might be useful as marker for bacteria living inside of marine snow particles.

Document Type: Article
Keywords: Marine Biology; marine environment; freshwater environment; hydrogen
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Kiel University
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1371/journal.pone.0013846
ISSN: 1932-6203
Projects: Future Ocean
Date Deposited: 22 Dec 2010 13:12
Last Modified: 05 Apr 2018 11:54
URI: http://oceanrep.geomar.de/id/eprint/10609

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