A new chemostat system to investigate combined temperature and CO2 effects on marine phytoplankton species.

Borchard, Corinna, Händel, N., Borges, A. V. and Engel, Anja (2009) A new chemostat system to investigate combined temperature and CO2 effects on marine phytoplankton species. [Talk] In: ASLO Aquatic Sciences Meeting 2009. , 25.-30.01.2009, Nice, France .

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Abstract

In order to better understand the consequences of global change on marine plankton and biogeochemical cycling we established a new continuous culture facility that enables the investigation of combined temperature and CO2 effects. The facility consists of five independent chemostats to cultivate algae in physiological steady-state under a controlled temperature, pCO2, nutrient and light regime. Each chemostat is surrounded by a water jacket that controls temperature within a deviation of 0.2°C. In the incubators, the cultures are continuously aerated with gas of preset CO2 concentrations. Distinct pCO2 conditions, ranging from 0 to more than 5000 µatm (± <1.8%) can be created using a two-step CO2 regulation system. To test the stability of the system and to asses the suitability for investigation of temperature and CO2 effects on phytoplankton, we followed the growth of a calcifying strain of Emiliania huxleyi under three different CO2 concentrations (180, 380 and 750 µatm) and two different temperatures (14ºC and 18ºC) simulating glacial, present day and future climate conditions. We will discuss the suitability of this system to investigate different scenarios of ocean warming and acidification.

Document Type: Conference or Workshop Item (Talk)
Keywords: Marine Biology; marine phytoplankton
Date Deposited: 07 Oct 2011 09:00
Last Modified: 21 May 2013 07:37
URI: http://oceanrep.geomar.de/id/eprint/12290

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