Cellular pH measurements in Emiliania huxleyi reveal pronounced membrane proton permeability.

Suffrian, Kerstin, Schulz, Kai G., Gutowska, Magdalena, Riebesell, Ulf and Bleich, Markus (2011) Cellular pH measurements in Emiliania huxleyi reveal pronounced membrane proton permeability. New Phytologist, 190 (3). pp. 595-608. DOI 10.1111/j.1469-8137.2010.03633.x.

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Abstract

To understand the influence of changing surface ocean pH and carbonate chemistry on the coccolithophore Emiliania huxleyi, it is necessary to characterize mechanisms involved in pH homeostasis and ion transport.
Here, we measured effects of changes in seawater carbonate chemistry on the fluorescence emission ratio of BCECF (2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein) as a measure of intracellular pH (pH(i)). Out of equilibrium solutions were used to differentiate between membrane permeation pathways for H+, CO(2) and HCO(3)-.
Changes in fluorescence ratio were calibrated in single cells, resulting in a ratio change of 0.78 per pH(i) unit. pH(i) acutely followed the pH of seawater (pH(e)) in a linear fashion between pH(e) values of 6.5 and 9 with a slope of 0.44 per pH(e) unit. pH(i) was nearly insensitive to changes in seawater CO(2) at constant pH(e) and HCO(3)-. An increase in extracellular HCO(3)- resulted in a slight intracellular acidification. In the presence of DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid), a broad-spectrum inhibitor of anion exchangers, E. huxleyi acidified irreversibly. DIDS slightly reduced the effect of pH(e) on pH(i).
The data for the first time show the occurrence of a proton permeation pathway in E. huxleyi plasma membrane. pH(i) homeostasis involves a DIDS-sensitive mechanism.

Document Type: Article
Additional Information: WOS:000289641600012
Keywords: Biological Oceanography; acid-base metabolism; BCECF (2'; 7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein); bicarbonate transport; dissolved inorganic carbon (DIC); DIDS (4; 4'-diisothiocyanatostilbene-2; 2'-disulfonic acid); Emiliania huxleyi; nigericin; pH homeostasis; HIGH-CALCIFYING STRAIN; MARINE-PHYTOPLANKTON; COCCOLITH FORMATION; CARBON ACQUISITION; CULTURE AGE; SEAWATER; CO2; CALCIFICATION; PHOTOPERIOD; BICARBONATE
Research affiliation: Kiel University > Faculty of Medicine > Institute of Physiology
OceanRep > The Future Ocean - Cluster of Excellence > FO-R08
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence > FO-R04
OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1111/j.1469-8137.2010.03633.x
ISSN: 0028-646X
Projects: BIOACID, Future Ocean
Date Deposited: 11 Jan 2012 14:44
Last Modified: 08 Mar 2017 10:29
URI: http://oceanrep.geomar.de/id/eprint/13416

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