Purification and Characterization of a κ-carrageenase from Marine Pseudoalteromonas sp. QY202.

Duan, Gao-fei, Su, Bei, Han, Feng and Yu, Wen-gong (2010) Purification and Characterization of a κ-carrageenase from Marine Pseudoalteromonas sp. QY202. Zhongguo-Haiyang-Daxue-xuebao = Periodical of Ocean University of China, 40 (3). pp. 95-100.

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Abstract

A marine bacterium Pseudoalteromonas sp.QY202 with high κ-carrageenase activity was isolated from the surface of Chondrus crispus.The κ-carrageenase was purified to electrophoretic homogeneity from the culture supernatant by a procedure of ammonium sulfate precipitation,desalting and DEAE-sepharose ion exchange chromatography,and the characterization of the enzyme was studied.The results show that the enzyme is purified 23.1 folds with a total recovery yield of 43.9% and gives a single band on SDS-PAGE with a molecular mass of 33.2 kDa.The optimum temperature and pH for enzyme activity are 40 ℃ and pH8.0,respectively.The enzyme is stable at temperatures below 40 ℃ and over a range of pH7.0-8.0.For κ-carrageenan,the enzyme gave a Km value of 1.6 mg/mL.The enzyme activity could be enhanced by the presence of Na+ and K+,whereas enormously inhibited by Hg^2+and Cu^2+.The main hydrolysis products of κ-carrageenan by the enzyme are κ-neocarradiaose and κ-neocarratetraose.

Document Type: Article
Additional Information: in Chinese
Keywords: Biogeochemistry; κcarrageenase, Pseudoaltermonas, purification, characterization
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BM Biogeochemical Modeling
Refereed: No
Open Access Journal?: No
ISSN: 1672-5174
Date Deposited: 04 Jun 2012 05:53
Last Modified: 22 Apr 2016 08:58
URI: http://oceanrep.geomar.de/id/eprint/14351

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