Direct Electron Microscopy Study on the Morphological Diversity of Bacteriophage Populations in Lake Plußsee.

Demuth, Jutta , Neve, Horst and Witzel, Karl-Paul (1993) Direct Electron Microscopy Study on the Morphological Diversity of Bacteriophage Populations in Lake Plußsee. Applied and Environmental Microbiology, 59 (10). pp. 3378-3384.

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Abstract

Direct electron microscopy of bacteriophages adsorbed to a carbon film without prior enrichment by specific host strains or concentration by physical or chemical methods was used to study the morphological diversity of natural bacteriophage assemblages in a North German lake. All samples contained a mixture of morphologically different tailed viruses, which were regarded as bacteriophages. Most of them had isometric heads and long noncontractile tails, belonging to morphotype B1 (Siphoviridae). In addition, members of morphotypes A1 (Myoviridae), B2 (Siphoviridae with elongated heads), and C1 (Podoviridae) were present in lower numbers. Only one cubic virus was detected, while no filamentous or pleomorphic phages were found. Up to 11 different phages per sample, and a total of 39 phages when all samples were considered together, could be distinguished by morphological criteria. The total number of phages was estimated to be on the order of 108/ml.

Document Type: Article
Additional Information: WOS:A1993MA35300031
Keywords: VIRUS-LIKE PARTICLES; MARINE-BACTERIA; AQUATIC ENVIRONMENTS; CYANOBACTERIA; ABUNDANCE; WATERS; PHYTOPLANKTON; MORTALITY; DYNAMICS; BAY
Refereed: Yes
Open Access Journal?: No
ISSN: 0099-2240
Date Deposited: 29 Oct 2012 14:06
Last Modified: 26 Apr 2016 10:28
URI: http://oceanrep.geomar.de/id/eprint/16574

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