Presence of Legionellaceae in warm water supplies and typing of strains by polymerase chain reaction.

Zietz, B., Wiese, Jutta , Brengelmann, F. and Dunkelberg, H. (2001) Presence of Legionellaceae in warm water supplies and typing of strains by polymerase chain reaction. Epidemiology and Infection, 126 (1). pp. 147-152.

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Abstract

Outbreaks of Legionnaire's disease present a public health challenge especially because fatal outcomes still remain frequent. The aim of this study was to describe the abundance and epidemiology of Legionellaceae in the human-made environment. Water was sampled from hot-water taps in private and public buildings across the area of Göttingen, Germany, including distant suburbs. Following isolation, we used polymerase chain reaction in order to generate strain specific banding profiles of legionella isolates. In total, 70 buildings were examined. Of these 18 (26%) had the bacterium in at least one water sample. Legionella pneumophila serogroups 1, 4, 5 and 6 could be identified in the water samples. Most of the buildings were colonized solely by one distinct strain, as proven by PCR. In three cases equal patterns were found in separate buildings. There were two buildings in this study where isolates with different serogroups were found at the same time.

Document Type: Article
Additional Information: WOS:000168097700020
Keywords: FIELD GEL-ELECTROPHORESIS; ARBITRARILY PRIMED PCR; PNEUMOPHILA SEROGROUP-1; LEGIONNAIRES DISEASE; POTABLE WATER; DNA; REPRODUCIBILITY; AMPLIFICATION; ENVIRONMENT; SYSTEMS
Refereed: Yes
Open Access Journal?: Yes
ISSN: 0950-2688
Date Deposited: 29 Oct 2012 13:52
Last Modified: 14 Sep 2018 08:01
URI: http://oceanrep.geomar.de/id/eprint/16579

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