An arabinogalactan-protein from whole grain of Avena sativa L. belongs to the wattle-blossom type of arabinogalactan-proteins.

Gollner, E. M., Ichinose, H., Kaneko, S., Blaschek, W. and Classen, Birgit (2011) An arabinogalactan-protein from whole grain of Avena sativa L. belongs to the wattle-blossom type of arabinogalactan-proteins. Journal of Cereal Science, 53 (2). pp. 244-249. DOI 10.1016/j.jcs.2011.01.004.

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Abstract

An arabinogalactan-protein (AGP) from whole grain of oat (Avena sativa L) has been isolated for the first time by double precipitation with beta-glucosyl Yariv reagent and characterized with regard to its polysaccharide and protein part. The large carbohydrate moiety is rich in galactose (63.0 w/w) and arabinose (32.8 w/w) and free of uronic acids. Linkage analysis of AGP and its products obtained by partial acid hydrolysis as well as enzymatic degradation with specific recombinant enzymes revealed that the carbohydrate moiety consists of a 1,3-Galp backbone and is linked in position 6 to short 1,6-Galp chains, terminating in Aral In the protein part, high contents of alanine, hydroxyproline and serine could be found which are typical for AGPs. The molecular mass of AGP was determined to be 83 kDa. The carbohydrate moieties, released by alkaline degradation of the protein part, had a size of about 7 kDa. Consequently, the overall structure of the AGP from oat could be assigned to be consistent with the wattle-blossom model of AGPs. (C) 2011 Elsevier Ltd. All rights reserved.

Document Type: Article
Keywords: Arabinogalactan-protein Avena sativa Enzymatic hydrolysis Yariv reagent streptomyces-avermitilis nbrc14893 water-extractable arabinogalactan wheat-flour echinacea-purpurea structural features alditol acetates yariv reagent peptide polysaccharides hydroxyproline
Research affiliation: Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
Publisher: Elsevier
Projects: Future Ocean
Date Deposited: 01 Nov 2012 05:01
Last Modified: 23 Sep 2019 22:24
URI: https://oceanrep.geomar.de/id/eprint/17335

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