Suitability of Vader for Transposon-Mediated Mutagenesis in Aspergillus niger.

Hihlal, E., Braumann, I., van den Berg, M. and Kempken, Frank (2011) Suitability of Vader for Transposon-Mediated Mutagenesis in Aspergillus niger. Applied and Environmental Microbiology, 77 (7). pp. 2332-2336. DOI 10.1128/aem.02688-10.

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Supplementary data:


The filamentous fungus Aspergillus niger is widely used in biotechnological applications. Strain CBS513.88 is known to harbor 21 copies of the nonautonomous transposon Vader. Upon selection of chlorate-resistant A. niger colonies, one Vader copy was found integrated in the nirA gene. This copy was used for vector construction and development of a transposon-tagging method. Vader showed an excision frequency of about 1 in 2.2 x 10(5) conidiospores. A total of 95 of 97 colonies analyzed exhibited an excision event at the DNA level, and Vader footprints were found. By employing thermal asymmetric interlaced (TAIL)-PCR, the reintegration sites of 21 independent excision events were determined. All reintegration events occurred within or very close to genes. Therefore, this method can be used for transposon mutagenesis in A. niger.

Document Type: Article
Keywords: penicillium-chrysogenum tolypocladium-inflatum filamentous fungi var awamori elements gene nidulans identification restless genome
Research affiliation: Kiel University
Refereed: Yes
DOI etc.: 10.1128/aem.02688-10
ISSN: 0099-2240
Date Deposited: 01 Nov 2012 04:58
Last Modified: 23 Jan 2013 10:04

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