Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget

Hüning, A., Melzner, Frank, Thomsen, F., Gutowska, Magdalena, Krämer, L., Rosenstiel, Philip, Pörtner, Hans-Otto, Philipp, E. E. R. and Lucassen, M. (2013) Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel; implications for shell formation and energy budget Marine Biology, 160 (8). pp. 1845-1861. DOI 10.1007/s00227-012-1930-9.

[img]
Preview
Text
227_2012_1930_MOESM1_ESM.pdf - Supplemental Material

Download (299Kb) | Preview
[img]
Preview
Text
227_2012_1930_MOESM2_ESM.pdf - Supplemental Material

Download (1116Kb) | Preview
[img]
Preview
Text
227_2012_1930_MOESM3_ESM.pdf - Supplemental Material

Download (418Kb) | Preview
[img] Text
art_10.1007_s00227-012-1930-9.pdf - Published Version
Restricted to Registered users only

Download (702Kb) | Contact

Supplementary data:

Abstract

Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mussel Mytilus edulis from the Baltic Sea. Based on the M. edulis mantle tissue transcriptome, the expression of several genes involved in metabolism, calcification and stress responses was assessed in the outer (marginal and pallial zone) and the inner mantle tissues (central zone) using quantitative real-time PCR. The expression of genes involved in energy and protein metabolism (F-ATPase, hexokinase and elongation factor alpha) was strongly affected by acclimation to moderately elevated CO2 partial pressures. Expression of a chitinase, potentially important for the calcification process, was strongly depressed (maximum ninefold), correlating with a linear decrease in shell growth observed in the experimental animals. Interestingly, shell matrix protein candidate genes were less affected by CO2 in both tissues. A compensatory process toward enhanced shell protection is indicated by a massive increase in the expression of tyrosinase, a gene involved in periostracum formation (maximum 220-fold). Using correlation matrices and a force-directed layout network graph, we were able to uncover possible underlying regulatory networks and the connections between different pathways, thereby providing a molecular basis of observed changes in animal physiology in response to ocean acidification.

Document Type: Article
Additional Information: WOS:000323066600008
Keywords: ocean acidification; Microbiology; Zoology; Freshwater & Marine Ecology; Marine & Freshwater Sciences; Oceanography
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Kiel University > Faculty of Medicine > Institute of Clinical Molecular Biology
Kiel University
Kiel University > Kiel Marine Science
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1007/s00227-012-1930-9
ISSN: 0025-3162
Projects: BIOACID, PACES, Future Ocean
Date Deposited: 07 Nov 2012 07:01
Last Modified: 12 Jan 2015 12:16
URI: http://oceanrep.geomar.de/id/eprint/19118

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...