Characterizing the Anaerobic Response of Chlamydomonas reinhardtii by Quantitative Proteomics
Terashima, Mia, Specht, Michael, Naumann, Bianca and Hippler, Michael (2010) Characterizing the Anaerobic Response of Chlamydomonas reinhardtii by Quantitative Proteomics Molecular & Cellular Proteomics, 9 (7). pp. 1514-1532. DOI 10.1074/mcp.M900421-MCP200.
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The versatile metabolism of the green alga Chlamydomonas reinhardtii is reflected in its complex response to anaerobic conditions. The anaerobic response is also remarkable in the context of renewable energy because C. reinhardtii is able to produce hydrogen under anaerobic conditions. To identify proteins involved during anaerobic acclimation as well as to localize proteins and pathways to the powerhouses of the cell, chloroplasts and mitochondria from C. reinhardtii in aerobic and anaerobic (induced by 8 h of argon bubbling) conditions were isolated and analyzed using comparative proteomics. A total of 2315 proteins were identified. Further analysis based on spectral counting clearly localized 606 of these proteins to the chloroplast, including many proteins of the fermentative metabolism. Comparative quantitative analyses were performed with the chloroplast-localized proteins using stable isotopic labeling of amino acids ([(13)C(6)] arginine/[(12)C(6)]arginine in an arginine auxotrophic strain). The quantitative data confirmed proteins previously characterized as induced at the transcript level as well as identified several new proteins of unknown function induced under anaerobic conditions. These proteins of unknown function provide new candidates for further investigation, which could bring insights for the engineering of hydrogen-producing alga strains
|Keywords:||ARABIDOPSIS-THALIANA CHLOROPLASTS; LIGHT-HARVESTING COMPLEX; PYRUVATE FORMATE-LYASE; PHOTOSYSTEM-II; MASS-SPECTROMETRY; GENE-EXPRESSION; FERMENTATIVE METABOLISM; BIOENERGETIC PATHWAYS; HYDROGEN-PRODUCTION; IRON-DEFICIENCY|
|Date Deposited:||13 Nov 2012 12:39|
|Last Modified:||05 May 2014 10:44|
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