Phylogenetic analysis and antibiotic activity of bacteria isolated from the surface of two co-occurring macroalgae from the Baltic Sea.

Goecke, Franz Ronald, Labes, Antje, Wiese, Jutta and Imhoff, Johannes F. (2013) Phylogenetic analysis and antibiotic activity of bacteria isolated from the surface of two co-occurring macroalgae from the Baltic Sea. European Journal of Phycology, 48 (1). pp. 47-60. DOI 10.1080/09670262.2013.767944.

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Abstract

Bacteria associated with Fucus vesiculosus and Delesseria sanguinea, two macroalgae from the Kiel Fjord were investigated seasonally over two years by cultivation-based methods. A total of 166 bacterial strains were isolated from the macroalgae, affiliated to seven classes of bacteria (Actinobacteria, Bacilli, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Cytophagia and Flavobacteria). According to 16S rRNA gene sequence similarities they were arranged in 82 phylotypes of>99.0% sequence identity. Assuming that chemical factors rule the bacteriamacroalga and bacteriabacteria interactions on algal surfaces, we tested the antibiotic activity of the bacterial isolates not only against a panel of four standard test organisms (Bacillus subtilis, Candida glabrata, Escherichia coli and Staphylococcus lentus) but also four macroalga-associated microorganisms: Algicola bacteriolytica and Pseudoalteromonas elyakovii (macroalgal pathogens), and Bacillus algicola and Formosa algae (strains associated with algal surfaces). Organic extracts of more than 51% of the isolates from the two macroalgae inhibited the growth of at least one of the tested microorganisms. As much as 46% and 45% of the isolates derived from F. vesiculosus and D. sanguinea, respectively, showed antimicrobial activity against the set of macroalga-associated bacteria, compared with 13 and 19% against a standard set of microorganisms. High antibacterial activity against macroalgal pathogens and bacterial competitors support the assumption that complex chemical interactions shape the relationships of bacteria associated with macroalgae and suggest that these bacteria are a rich source of antimicrobial metabolites.

Document Type: Article
Additional Information: WOS:000316444400004
Keywords: antimicrobial activities; bacterial cultures; chemical associations; Delesseria; epiphytic bacteria; Fucus; Kiel; macroalgabacteria associations
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > GEOMAR > Applied R&D > Centre for Marine Substances (KiWiZ)
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1080/09670262.2013.767944
ISSN: 0967-0262
Projects: KIWIZ, Future Ocean
Date Deposited: 06 Dec 2012 10:48
Last Modified: 04 Jan 2017 12:52
URI: http://oceanrep.geomar.de/id/eprint/19592

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