Retention of a transgenerational marker ((137)Barium) in tissues of adult female anemonefish and assessment of physiological stress.

Roy, Alexandra-Sophie, Frisch, Ashley J., Syms, Craig, Thorrold, Simon R. and Jones, Geoffrey P. (2013) Retention of a transgenerational marker ((137)Barium) in tissues of adult female anemonefish and assessment of physiological stress. Environmental Biology of Fishes, 96 (4). pp. 459-466. DOI 10.1007/s10641-012-0029-y.

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Abstract

Recently it was shown that female fish injected with enriched stable isotopes maternally transmit a chemical signature to larval otoliths. Validation of this larval marking technique requires laboratory experiments to determine appropriate injection concentrations and to assess any negative effects on larval and adult condition. This study investigated the temporal profile of (137)barium assimilation and retention in tissues of adult female anemonefish Amphiprion melanopus (Pomacentridae) following intraperitoneal injection with either 2 or 4 mu g Ba-137 g(-1) body mass. Mean barium isotope ratios (Ba-138:Ba-137) in the two groups of treated fish were not significantly different from each other, but were significantly different from those in the control group up to 56 days post-injection. This pattern of Ba-137 retention was consistent across gonad, muscle, liver and bone tissues. Mean plasma cortisol concentration (an indicator of non-specific physiological stress) was not significantly different among groups and was considered to be representative of unstressed fish. Together, these results indicate that (1) A. melanopus suffer minimal physiological stress and cope well after treatment with Ba-137, (2) Ba-137 is retained in female A. melanopus for a prolonged period (at least 56 days), such that multiple clutches of offspring are likely to be marked with an isotopic signature, and (3) a lower dosage of 2 mu g Ba-137 g(-1) appears sufficient for transgenerational marking. It is concluded that Ba-137 is suitable for use as a transgenerational marker and is a powerful tool to resolve long-standing enigmas such as larval dispersal distances and the fishery benefits of marine reserves.

Document Type: Article
Additional Information: WOS:000315595200004
Keywords: Amphiprion melanopus; Coral reef fish; Larval tagging; Batch marking; Otolith microchemistry; Plasma cortisol; management; trout plectropomus-leopardus; marine population; stable isotopes; responses; connectivity; marking
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1007/s10641-012-0029-y
ISSN: 0378-1909
Date Deposited: 04 Apr 2013 05:42
Last Modified: 22 May 2017 11:43
URI: http://oceanrep.geomar.de/id/eprint/20826

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