Dietary β-glucan stimulate complement and C-reactive protein acute phase responses in common carp (Cyprinus carpio) during an Aeromonas salmonicida infection.

Pionnier, Nicolas, Falco, Alberto, Miest, Joanna J., Frost, Patrick, Irnazarow, Ilgiz, Shrive, Annette and Hoole, Dave (2013) Dietary β-glucan stimulate complement and C-reactive protein acute phase responses in common carp (Cyprinus carpio) during an Aeromonas salmonicida infection. Fish & Shellfish Immunology, 34 (3). pp. 819-831. DOI 10.1016/j.fsi.2012.12.017.

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Abstract

The effect of beta-glucans as feed additive on the profile of C-reactive protein (CRP) and complement acute phase responses was studied in common carp Cyprinus carpio after exposition to a bacterial infection with Aeromonas salmonicida. Carp were orally administered with beta-glucan (MacroGard (R)) for 14 days with a daily beta-glucan intake of 6 mg per kg body weight. Fish were then intraperitoneally injected with either PBS or 1 x 10(8) bacteria per fish and sampled at time 0, 6, 12, 24, 48, 72, 96 and 120 h post-injection (p.i.) for serum and head kidney, liver and mid-gut tissues. CRP levels and complement activity were determined in the serum samples whilst the gene expression profiles of CRP and complement related genes (crp1, crp2, c1r/s, bf/c2, c3 and masp2) were analysed in the tissues by quantitative PCR. Results obtained showed that oral administration of beta-glucan for 14 days significantly increased serum CRP levels up to 2 fold and serum alternative complement activity (ACP) up to 35 fold. The bacterial infection on its own (i.e. not combined with a beta-glucan feeding) did have significant effects on complement response whilst CRP was not detectably induced during the carp acute phase reaction. However, the combination of the infection and the beta-glucan feeding did show significant effects on both CRP and complement profiles with higher serum CRP levels and serum ACP activity in the beta-glucan fed fish than in the control fed fish. In addition, a distinct organ and time dependent expression profile pattern was detected for all the selected genes: a peak of gene expression first occurred in the head kidney tissue (6 h p.i. or 12 h p.i.), then an up-regulation in the liver several hours later (24 h p.i.) and finally up- or down-regulations in the mid-gut at 24 h p.i. and 72 h p.i. In conclusion, the results of this study suggest that MacroGard (R) stimulated CRP and complement responses to A. salmonicida infection in common carp.

Document Type: Article
Keywords: Beta-glucan; C-reactive protein; Complement; Aeromonas salmonicida; Cyprinus carpi
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.fsi.2012.12.017
ISSN: 1050-4648
Date Deposited: 08 Jul 2013 07:11
Last Modified: 08 Jul 2013 07:11
URI: http://oceanrep.geomar.de/id/eprint/21516

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