Early stimulation of the immune system of an important aquaculture fish species: Probiotic application in European sea bass juveniles.

Franke, Andrea, Roth, Olivia and Clemmesen, Catriona (2013) Early stimulation of the immune system of an important aquaculture fish species: Probiotic application in European sea bass juveniles. Fish & Shellfish Immunology, 34 (6). p. 1707. DOI 10.1016/j.fsi.2013.03.214.

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Abstract

The high mortality during fish early life stages is a major bottleneck in aquaculture. Therefore, the establishment of methods to prevent and control diseases, to ensure efficient growth and to reach maximal survival rates is mandatory to optimize the productivity. A promising solution can be the early activation of the immune system by administration of probiotics as nutritional supplements.

In our study we assess the effect of the probiotic candidate Bacillus subtilis on the innate and adaptive immune response of juvenile European sea bass (Dicentrarchus labrax). Therefore, Artemia nauplii were used as live carriers to feed B. subtilis to 3-month-old sea bass over a period of 2 weeks. Subsequently, the juveniles were fed another week without administering B. subtilis in order to estimate the bacterial mucus-binding ability. During the course of the experiment, we evaluated direct effects on the cellular immune response by fluorescence-activated cell sorting analysis and on survival. As a next step we will determine profiles of immune gene expression. To estimate cellular stress, the expression level of metabolism- and stress-related genes will be measured. Furthermore, the RNA/DNA ratio as an indicator of growth will be analysed.

Document Type: Article
Additional Information: WOS:000319646100237 Meeting abstract
Keywords: aquaculture; sea bass
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.fsi.2013.03.214
ISSN: 1050-4648
Projects: Future Ocean
Date Deposited: 08 Nov 2013 09:33
Last Modified: 23 Sep 2019 20:50
URI: http://oceanrep.geomar.de/id/eprint/22371

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