FoxO is a critical regulator of stem cell maintenance in immortal Hydra.

Boehm, A. M., Khalturin, K., Anton-Erxleben, F., Hemmrich, G., Klostermeier, U. C., Lopez-Quintero, J. A., Oberg, H. H., Puchert, M., Rosenstiel, Philip, Wittlieb, J. and Bosch, Thomas (2012) FoxO is a critical regulator of stem cell maintenance in immortal Hydra. PNAS Proceedings of the National Academy of Sciences of the United States of America, 109 (48). pp. 19697-19702. DOI 10.5214/ans.0972.7531.200107.

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Supplementary data:

Abstract

Hydra's unlimited life span has long attracted attention from natural scientists. The reason for that phenomenon is the indefinite self-renewal capacity of its stem cells. The underlying molecular mechanisms have yet to be explored. Here, by comparing the transcriptomes of Hydra's stem cells followed by functional analysis using transgenic polyps, we identified the transcription factor forkhead box O (FoxO) as one of the critical drivers of this continuous self-renewal. foxO overexpression increased interstitial stem cell and progenitor cell proliferation and activated stem cell genes in terminally differentiated somatic cells. foxO down-regulation led to an increase in the number of terminally differentiated cells, resulting in a drastically reduced population growth rate. In addition, it caused down-regulation of stem cell genes and antimicrobial peptide (AMP) expression. These findings contribute to a molecular understanding of Hydra's immortality, indicate an evolutionarily conserved role of FoxO in controlling longevity from Hydra to humans, and have implications for understanding cellular aging.

Document Type: Article
Additional Information: Univ Kiel, Zool Inst, D-24098 Kiel, Germany. Univ Hosp Schleswig Holstein, Inst Clin Mol Biol IKMB, D-24105 Kiel, Germany. Univ Hosp Schleswig Holstein, Inst Immunol, D-24105 Kiel, Germany. Bosch, TCG (reprint author), Univ Kiel, Zool Inst, D-24098 Kiel, Germany. tbosch@zoologie.uni-kiel.de
Keywords: adult stem cell differentiation transcription factors caenorhabditis-elegans bayesian-inference homeostasis longevity evolution differentiation association nematocyst genomics
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.5214/ans.0972.7531.200107
ISSN: 0027-8424
Projects: Future Ocean
Date Deposited: 14 May 2014 10:21
Last Modified: 19 Aug 2019 10:08
URI: http://oceanrep.geomar.de/id/eprint/23839

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