Conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and delta B-11

Heinemann, Agnes, Fietzke, Jan, Melzner, Frank, Böhm, Florian, Thomsen, Jörn, Garbe-Schönberg, Dieter and Eisenhauer, Anton (2012) Conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and delta B-11 Geochemistry Geophysics Geosystems, 13 (1). Q01005. DOI 10.1029/2011GC003790.

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Abstract

Mytilus edulis were cultured for 3 months under six different seawater pCO(2) levels ranging from 380 to 4000 mu atm. Specimen were taken from Kiel Fjord (Western Baltic Sea, Germany) which is a habitat with high and variable seawater pCO(2) and related shifts in carbonate system speciation (e. g., low pH and low CaCO3 saturation state). Hemolymph (HL) and extrapallial fluid (EPF) samples were analyzed for pH and total dissolved inorganic carbon (C-T) to calculate pCO(2) and [HCO3-]. A second experiment was conducted for 2 months with three different pCO(2) levels (380, 1400 and 4000 mu atm). Boron isotopes (delta B-11) were investigated by LA-MC-ICP-MS (Laser Ablation-Multicollector-Inductively Coupled Plasma-Mass Spectrometry) in shell portions precipitated during experimental treatment time. Additionally, elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF of specimen from the second experiment were measured via ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). Extracellular pH was not significantly different in HL and EPF but systematically lower than ambient water pH. This is due to high extracellular pCO(2) values, a prerequisite for metabolic CO2 excretion. No accumulation of extracellular [HCO3-] was measured. Elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF increased slightly with pH which is in accordance with increasing growth and calcification rates at higher seawater pH values. Boron isotope ratios were highly variable between different individuals but also within single shells. This corresponds to a high individual variability in fluid B/Ca ratios and may be due to high boron concentrations in the organic parts of the shell. The mean delta B-11 value shows no trend with pH but appears to represent internal pH (EPF) rather than ambient water pH.

Document Type: Article
Additional Information: WOS:000299176800001
Keywords: mytilus edulis; acid base; boron isotopes; extrapallial fluid; ocean acidification; trace metals; ocean acidification; calcification rate pecten-maximus; seawater acidification biological-controls; anthropogenic co2
Research affiliation: Kiel University > Faculty of Mathematics and Natural Sciences > Institute of Geosciences
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-MG Marine Geosystems
OceanRep > The Future Ocean - Cluster of Excellence > FO-R09
OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Refereed: Yes
DOI etc.: 10.1029/2011GC003790
ISSN: 1525-2027
Projects: Future Ocean, BIOACID
Date Deposited: 14 May 2014 10:03
Last Modified: 27 Feb 2017 15:45
URI: http://oceanrep.geomar.de/id/eprint/24033

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