Gene expression changes in the coccolithophore Emiliania huxleyi after 500 generations of selection to ocean acidification

Lohbeck, Kai T., Riebesell, Ulf and Reusch, Thorsten B.H. (2014) Gene expression changes in the coccolithophore Emiliania huxleyi after 500 generations of selection to ocean acidification Proceedings of the Royal Society B-Biological Sciences, 281 (1786). p. 20140003. DOI 10.1098/rspb.2014.0003.

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Text (Genes of interest (GOI) and endogenous reference genes (ERGs) used in this study )
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Text ( Multivariate analyses of variance on –ΔCT data from ambient, medium and high CO2 adapted Emiliania huxleyi populations)
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Abstract

Coccolithophores are unicellular marine algae that produce biogenic calcite scales and substantially contribute to marine primary production and carbon export to the deep ocean. Ongoing ocean acidification particularly impairs calcifying organisms, mostly resulting in decreased growth and calcification. Recent studies revealed that the immediate physiological response in the coccolithophore Emiliania huxleyi to ocean acidification may be partially compensated by evolutionary adaptation, yet the underlying molecular mechanisms are currently unknown. Here, we report on the expression levels of 10 candidate genes putatively relevant to pH regulation, carbon transport, calcification and photosynthesis in E. huxleyi populations short-term exposed to ocean acidification conditions after acclimation (physiological response) and after 500 generations of high CO2 adaptation (adaptive response). The physiological response revealed downregulation of candidate genes, well reflecting the concomitant decrease of growth and calcification. In the adaptive response, putative pH regulation and carbon transport genes were up-regulated, matching partial restoration of growth and calcification in high CO2-adapted populations. Adaptation to ocean acidification in E. huxleyi likely involved improved cellular pH regulation, presumably indirectly affecting calcification. Adaptive evolution may thus have the potential to partially restore cellular pH regulatory capacity and thereby mitigate adverse effects of ocean acidification.

Document Type: Article
Additional Information: WOS:000336784500009
Keywords: adaptation; calcification; gene expression; ocean acidification; phytoplankton
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Evolutionary Ecology of Marine Fishes
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Refereed: Yes
DOI etc.: 10.1098/rspb.2014.0003
ISSN: 0962-8452
Projects: BIOACID
Date Deposited: 16 May 2014 07:54
Last Modified: 24 Oct 2016 12:56
URI: http://oceanrep.geomar.de/id/eprint/24557

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