Feeding common carp Cyprinus carpio with β-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection.

Pionnier, Nicolas, Falco, Alberto, Miest, Joanna J., Shrive, Annette K. and Hoole, Dave (2014) Feeding common carp Cyprinus carpio with β-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection. Fish & Shellfish Immunology, 39 (2). pp. 285-295. DOI 10.1016/j.fsi.2014.05.008.

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Supplementary data:

Abstract

Highlights:
• Feeding 0.1% Macrogard® for 25 days increased CRP and complement responses in carp.
• β-glucan affected gene expression in the liver, head kidney and mid-gut tissues.
• Gene expression was more affected by LPS and poly(I:C) in the β-glucan fed fish.
• Carp acute phase response to PAMPs injection was stimulated by β-glucan.

Abstract:
The effect of β-glucan as a feed additive on the serum and gene profile of C-reactive protein (CRP) and complement acute phase responses was ascertained in common carp Cyprinus carpio. In addition effects of subsequent intraperitoneal injections of pathogen-associated molecular patterns (PAMPs), i.e. LPS or poly(I:C), to mimic bacterial or viral infection respectively, were studied. Carp were first orally fed with β-glucan (MacroGard®) with a daily β-glucan intake of 6 mg per kg body weight or with control food for 25 days and then injected with PBS containing either LPS (4 mg/kg) or poly(I:C) (5 mg/kg) or PBS alone. Fish were sampled during the 25 days of the feeding period and up to 7 days post-PAMPs injections for serum and liver, head kidney and mid-gut tissues. Oral administration of β-glucan for 25 days significantly increased serum CRP levels and alternative complement activity (ACP). In addition, the subsequent LPS and poly(I:C) challenges significantly affected CRP and complement related gene expression profiles (crp1, crp2, c1r/s, bf/c2, c3 and masp2), with the greatest effects observed in the β-glucan fed fish. However, in fish fed β-glucan the PAMPs injections had less effects on CRP levels and complement activity in the serum than in control fed fish, suggesting that the 25 days of β-glucan immunostimulation was sufficient enough to reduce the effects of LPS and poly(I:C) injections. Results suggest that MacroGard® stimulated CRP and complement responses to PAMPs immunological challenges in common carp thus highlighting the beneficial β-glucan immunostimulant properties.

Document Type: Article
Keywords: C-reactive protein; Complement; β-glucan; Immunostimulation; PAMPs; LPS; Poly(I:C); Cyprinus carpio
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.fsi.2014.05.008
ISSN: 1050-4648
Date Deposited: 26 May 2014 13:11
Last Modified: 05 Jan 2017 13:28
URI: http://oceanrep.geomar.de/id/eprint/24632

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