New highly fluorescent pH indicator for ratiometric RGB imaging of pCO2.

Schutting, Susanne, Klimant, Ingo, de Beer, Dirk and Borisov, Sergey M. (2014) New highly fluorescent pH indicator for ratiometric RGB imaging of pCO2. Methods and Applications in Fluorescence, 2 (2). 024001. DOI 10.1088/2050-6120/2/2/024001.

[thumbnail of 2050-6120_2_2_024001.pdf] Text
2050-6120_2_2_024001.pdf - Published Version
Available under License Creative Commons Attribution.

Download (422kB)
[thumbnail of maf_2_2_024001_suppdata.pdf]
Preview
Text
maf_2_2_024001_suppdata.pdf - Supplemental Material
Available under License Creative Commons Attribution.

Download (89kB) | Preview

Supplementary data:

Abstract

A new diketo-pyrrolo-pyrrole (DPP) indicator dye for optical sensing of carbon dioxide is prepared via a simple one step synthesis from commercially available low cost 'Pigment Orange 73'. The pigment is modified via alkylation of one of the lactam nitrogens with a tert-butylbenzyl group. The indicator dye is highly soluble in organic solvents and in polymers and shows pH-dependent absorption (λmax 501 and 572 nm for the protonated and deprotonated forms, respectively) and emission spectra (λmax 524 and 605 nm for the protonated and deprotonated forms, respectively). Both the protonated and the deprotonated forms show high fluorescence quantum yields (Φprot 0.86; Φdeprot 0.66). Hence, colorimetric read-out and ratiometric fluorescence intensity measurements are possible. The emission of the two forms of the indicator excellently matches the response of the green and the red channels of an RGB camera. This enables imaging of carbon dioxide distribution with a simple and low cost optical set-up. The sensor based on the new DPP dye shows very high sensitivity and is particularly promising for monitoring atmospheric levels of carbon dioxide.

Document Type: Article
Keywords: optical sensor, fluorescence, absorption, carbon dioxide, imaging
Refereed: Yes
Open Access Journal?: Yes
Publisher: IOP Publishing
Projects: ECO2, BIOACID
Date Deposited: 17 Jun 2014 09:26
Last Modified: 29 Apr 2016 12:30
URI: https://oceanrep.geomar.de/id/eprint/24752

Actions (login required)

View Item View Item