Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population.

Bagheri, H., El-Soda, M., Kim, H. K., Fritsche, S., Jung, Christian and Aarts, M. G. M. (2013) Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population. International Journal of Molecular Sciences, 14 (8). pp. 15561-15577. DOI 10.3390/ijms140815561.

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Supplementary data:

Abstract

The genetic basis of the wide variation for nutritional traits in Brassica rapa is largely unknown. A new Recombinant Inbred Line (RIL) population was profiled using High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) analysis to detect quantitative trait loci (QTLs) controlling seed tocopherol and seedling metabolite concentrations. RIL population parent L58 had a higher level of glucosinolates and phenylpropanoids, whereas levels of sucrose, glucose and glutamate were higher in the other RIL population parent, R-o-18. QTL related to seed tocopherol (α-, β-, γ-, δ-, α-⁄γ- and total tocopherol) concentrations were detected on chromosomes A3, A6, A9 and A10, explaining 11%–35% of the respective variation. The locus on A3 co-locates with the BrVTE1gene, encoding tocopherol cyclase. NMR spectroscopy identified the presence of organic/amino acid, sugar/glucosinolate and aromatic compounds in seedlings. QTL positions were obtained for most of the identified compounds. Compared to previous studies, novel loci were found for glucosinolate concentrations. This work can be used to design markers for marker-assisted selection of nutritional compounds in B. rapa.

Document Type: Article
Keywords: Brassica rapa; secondary metabolites; nutritional value; Quantitative Trait Loci; tocopherol; glucosinolates
Research affiliation: Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.3390/ijms140815561
ISSN: 1422-0067
Projects: Future Ocean
Date Deposited: 08 Jul 2014 08:50
Last Modified: 23 Sep 2019 23:34
URI: http://oceanrep.geomar.de/id/eprint/24812

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