Sulforaphane and phenylethyl isothiocyanate protect human skin from UV-induced inflammation and apoptosis.

Ernst, I. M., Kleszczynski, K., Wagner, A. E., Kruse, N., Zillikens, D., Rimbach, Gerald and Fischer, W. (2013) Sulforaphane and phenylethyl isothiocyanate protect human skin from UV-induced inflammation and apoptosis. Experimental Dermatology, 22 (3). E19-E19. DOI 10.1016/j.phrs.2013.09.009.

Full text not available from this repository.

Supplementary data:

Abstract

Chronic UVR-exposure may impair the stress response and antioxidant defense mechanisms of human skin. The transcription factor nuclear factor erythroid-2 related factor 2 (Nrf2) orchestrates the expression of genes coding for the stress response and antioxidant proteins. Here, we tested sulforaphane (SFN) and phenylethyl isothiocyanate (PEITC) for their ability to counteract UVR-induced oxidative stress and apoptosis in ex vivo human full-thickness skin combined with in vitro HaCaT keratinocytes. Investigation of Nrf2 transactivation and induction of genes coding for Nrf2-dependent phase II antioxidative enzymes (γ-glutamylcysteine-synthetase (γGCS), heme oxygenase 1 (HO-1) and NAD(P)H quinone oxidoreductase 1 (NQO1)) was performed in HaCaT keratinocytes. Comparative investigations in human ex vivo skin were conducted for analysis of gene expression of above mentioned phase II enzymes and catalase (CAT) as well as hematoxylin/eosin (H&E) and immunofluorescence (catalase, cleaved Casp-3). UVR exposure of human skin (300 mJ/cm2) resulted in a significant time-dependent increase of the number of sunburn cells and caspase-3 activation as biomarkers of apoptosis for up to 48 h (p < 0.001) and induced a significant decrease of the antioxidant enzyme catalase (p < 0.001). This was significantly counteracted by the pre-treatment of human skin with SFN and PEITC (5 μM and 10 μM). Mechanistic cell culture studies revealed SFN and PEITC to increase Nrf2 activity and Nrf2-dependent gene expression (γGCS, HO-1, NQO1); this was paralleled in human full skin mRNA. In conclusion, the induction of Nrf2-dependent antioxidant pathways seems to be a potential mechanism by which SFN and PEITC protect against UVR-induced oxidative stress and apoptosis in human skin.

Document Type: Article
Additional Information: Times Cited: 0 Ernst, I. M. Kleszczynski, K. Wagner, A. E. Kruse, N. Zillikens, D. Rimbach, G. Fischer, W. 40th Annual Meeting of the Association-of-Dermatological-Research Mar 14-16, 2013 Dessau, GERMANY
Keywords: Sulforaphane, Phenylethyl isothiocyanate, Nrf2, Skin, Apoptosis, UVR, Oxidative stress
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.phrs.2013.09.009
ISSN: 0906-6705
Projects: Future Ocean
Date Deposited: 08 Jul 2014 09:10
Last Modified: 23 Aug 2019 09:34
URI: http://oceanrep.geomar.de/id/eprint/24906

Actions (login required)

View Item View Item