Epigenetic Regulation of Depot-Specific Gene Expression in Adipose Tissue.

Gehrke, S., Brueckner, B., Schepky, A., Klein, J., Iwen, A., Bosch, T. C. G., Wenck, H., Winnefeld, M. and Hagemann, S. (2013) Epigenetic Regulation of Depot-Specific Gene Expression in Adipose Tissue. Open Access PLoS ONE, 8 (12). DOI 10.1371/journal.pone.0082516.

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Supplementary data:

Abstract

In humans, adipose tissue is distributed in subcutaneous abdominal and subcutaneous gluteal depots that comprise a variety of functional differences. Whereas energy storage in gluteal adipose tissue has been shown to mediate a protective effect, an increase of abdominal adipose tissue is associated with metabolic disorders. However, the molecular basis of depot-specific characteristics is not completely understood yet. Using array-based analyses of transcription profiles, we identified a specific set of genes that was differentially expressed between subcutaneous abdominal and gluteal adipose tissue. To investigate the role of epigenetic regulation in depot-specific gene expression, we additionally analyzed genome-wide DNA methylation patterns in abdominal and gluteal depots. By combining both data sets, we identified a highly significant set of depot-specifically expressed genes that appear to be epigenetically regulated. Interestingly, the majority of these genes form part of the homeobox gene family. Moreover, genes involved in fatty acid metabolism were also differentially expressed. Therefore we suppose that changes in gene expression profiles might account for depot-specific differences in lipid composition. Indeed, triglycerides and fatty acids of abdominal adipose tissue were more saturated compared to triglycerides and fatty acids in gluteal adipose tissue. Taken together, our results uncover clear differences between abdominal and gluteal adipose tissue on the gene expression and DNA methylation level as well as in fatty acid composition. Therefore, a detailed molecular characterization of adipose tissue depots will be essential to develop new treatment strategies for metabolic syndrome associated complications.

Document Type: Article
Additional Information: Times Cited: 1 Gehrke, Sandra Brueckner, Bodo Schepky, Andreas Klein, Johannes Iwen, Alexander Bosch, Thomas C. G. Wenck, Horst Winnefeld, Marc Hagemann, Sabine
Keywords: DNA methylation Gene expression Adipose tissue Homeobox Fats Fatty acids Gene regulation Epigenetics
Research affiliation: Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1371/journal.pone.0082516
ISSN: 1932-6203
Projects: Future Ocean
Date Deposited: 08 Jul 2014 09:19
Last Modified: 23 Sep 2019 20:09
URI: http://oceanrep.geomar.de/id/eprint/24929

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