Genome-wide transcriptomic responses of the seagrasses Zostera marina and Nanozostera noltii under a simulated heatwave confirm functional types.

Franssen, Susanne U., Gu, Jenny, Winters, Gidon, Huylmans, Ann-Kathrin, Wienpahl, Isabell, Sparwel, Maximiliane, Coyer, James A., Olsen, Jeanine L., Reusch, Thorsten B.H. and Bornberg-Bauer, Erich (2014) Genome-wide transcriptomic responses of the seagrasses Zostera marina and Nanozostera noltii under a simulated heatwave confirm functional types. Open Access Marine Genomics, 15 . pp. 65-73. DOI 10.1016/j.margen.2014.03.004.

[img]
Preview
Text
1-s2.0-S1874778714000300-main.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1724Kb) | Preview
[img] Text (Supplementary figures S1–S9, supplementary tables S1–S4 and additional information on the transcriptome assembly for N. noltii.)
mmc1.docx - Supplemental Material
Available under License Creative Commons Attribution.

Download (1344Kb)
[img] Other (List of all 8977 investigated genes including their functional annotation and the information whether they are differentially expressed with respect to a certain factor (FDR α < 0.05).)
mmc2.xlsx - Supplemental Material
Available under License Creative Commons Attribution.

Download (1801Kb)

Supplementary data:

Abstract

Genome-wide transcription analysis between related species occurring in overlapping ranges can provide insights into the molecular basis underlying different ecological niches. The co-occurring seagrass species, Zostera marina and Nanozostera noltii, are found in marine coastal environments throughout the northern hemisphere. Z. marina is often dominant in subtidal environments and subjected to fewer temperature extremes compared to the predominately intertidal and more stress-tolerant N. noltii.

We exposed plants of both species to a realistic heat wave scenario in a common-stress-garden experiment. Using RNA-seq (~ 7 million reads/library), four Z. marina and four N. noltii libraries were compared representing northern (Denmark) and southern (Italy) locations within the co-occurring range of the species' European distribution.

A total of 8977 expressed genes were identified, of which 78 were directly related to heat stress. As predicted, both species were negatively affected by the heat wave, but showed markedly different molecular responses. In Z. marina the heat response was similar across locations in response to the heatwave at 26 °C, with a complex response in functions related to protein folding, synthesis of ribosomal chloroplast proteins, proteins involved in cell wall modification and heat shock proteins (HSPs). In N. noltii the heat response markedly differed between locations, while HSP genes were not induced in either population.

Our results suggest that as coastal seawater temperatures increase, Z. marina will disappear along its southern most ranges, whereas N. noltii will continue to move north. As a consequence, sub- and intertidal habitat partitioning may weaken in more northern regions because the higher thermal tolerance of N. noltii provides a competitive advantage in both habitats. Although previous studies have focused on HSPs, the present study clearly demonstrates that a broader examination of stress related genes is necessary.

Document Type: Article
Additional Information: Data accessibility Raw reads of 454 and Illumina sequencing are accessible at NCBI SRA (accession number of the complete study: SRP022957 including two 454 and eight Illumina libraries). The de novo assembly of the N. noltii transcriptome is available at: http://drzompo.uni-muenster.de/downloads, library: Nano_A.
Keywords: Inter-species transcriptomics; RNA-seq; Zostera marina & Nanozostera noltii; Heatwave; Intra-specific variability; Global warming
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.margen.2014.03.004
ISSN: 1874-7787
Related URLs:
Projects: AQUASHIFT
Date Deposited: 27 Jun 2014 11:27
Last Modified: 23 Aug 2017 07:07
URI: http://oceanrep.geomar.de/id/eprint/24980

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...