Characterization of bud emergence 46 (BEM46) protein: Sequence, structural, phylogenetic and subcellular localization analyses.

Kumar, Abhishek, Kollath-Leiss, K. and Kempken, Frank (2013) Characterization of bud emergence 46 (BEM46) protein: Sequence, structural, phylogenetic and subcellular localization analyses. Biochemical and Biophysical Research Communications, 438 (3). pp. 526-532. DOI 10.1016/j.bbrc.2013.07.103.

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Supplementary data:

Abstract

The bud emergence 46 (BEM46) protein from Neurospora crassa belongs to the α/β-hydrolase superfamily. Recently, we have reported that the BEM46 protein is localized in the perinuclear ER and also forms spots close by the plasma membrane. The protein appears to be required for cell type-specific polarity formation in N. crassa. Furthermore, initial studies suggested that the BEM46 amino acid sequence is conserved in eukaryotes and is considered to be one of the widespread conserved “known unknown” eukaryotic genes. This warrants for a comprehensive phylogenetic analysis of this superfamily to unravel origin and molecular evolution of these genes in different eukaryotes. Herein, we observe that all eukaryotes have at least a single copy of a bem46 ortholog. Upon scanning of these proteins in various genomes, we find that there are expansions leading into several paralogs in vertebrates. Usingcomparative genomic analyses, we identified insertion/deletions (indels) in the conserved domain of BEM46 protein, which allow to differentiate fungal classes such as ascomycetes from basidiomycetes. We also find that exonic indels are able to differentiate BEM46 homologs of different eukaryotic lineage. Furthermore, we unravel that BEM46 protein from N. crassa possess a novel endoplasmic-retention signal (PEKK) using GFP-fusion tagging experiments. We propose that three residues namely a serine 188S, a histidine 292H and an aspartic acid 262D are most critical residues, forming a catalytic triad in BEM46 protein from N. crassa. We carried out a comprehensive study on bem46 genes from a molecular evolution perspective with combination of functional analyses. The evolutionary history of BEM46 proteins is characterized by exonic indels in lineage specific manner.

Document Type: Article
Additional Information: Times Cited: 0 Kumar, Abhishek Kollath-Leiss, Krisztina Kempken, Frank
Keywords: Bud emergence 46 (bem46), Evolution, Fungi, Indels, Endoplasmic retention signal, eGFP-Fusion, α/β-hydrolase
Research affiliation: Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.bbrc.2013.07.103
ISSN: 0006-291X
Projects: Future Ocean
Date Deposited: 08 Jul 2014 09:29
Last Modified: 23 Sep 2019 22:31
URI: http://oceanrep.geomar.de/id/eprint/25042

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