Aquatic Respiration Rate Measurements at Low Oxygen Concentrations.

Holtappels, Moritz, Tiano, Laura, Kalvelage, Tim, Lavik, Gaute, Revsbech, Niels Peter and Kuypers, Marcel M. M. (2014) Aquatic Respiration Rate Measurements at Low Oxygen Concentrations. Open Access PLoS ONE, 9 (2). e89369. DOI 10.1371/journal.pone.0089369.

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Abstract

Despite its huge ecological importance, microbial oxygen respiration in pelagic waters is little studied, primarily due to methodological difficulties. Respiration measurements are challenging because of the required high resolution of oxygen concentration measurements. Recent improvements in oxygen sensing techniques bear great potential to overcome these limitations. Here we compare 3 different methods to measure oxygen consumption rates at low oxygen concentrations, utilizing amperometric Clark type sensors (STOX), optical sensors (optodes), and mass spectrometry in combination with 18-18O2 labeling. Oxygen concentrations and consumption rates agreed well between the different methods when applied in the same experimental setting. Oxygen consumption rates between 30 and 400 nmol L−1 h−1 were measured with high precision and relative standard errors of less than 3%. Rate detection limits in the range of 1 nmol L−1 h−1 were suitable for rate determinations in open ocean water and were lowest at the lowest applied O2 concentration.

Document Type: Article
Research affiliation: OceanRep > SFB 754 > B4
OceanRep > SFB 754
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1371/journal.pone.0089369
ISSN: 1932-6203
Projects: SFB754
Date Deposited: 11 Aug 2014 10:49
Last Modified: 11 Aug 2014 10:49
URI: http://oceanrep.geomar.de/id/eprint/25428

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