Handling Temperature Bursts Reaching 464 C: Different Microbial Strategies in the Sisters Peak Hydrothermal Chimney.

Perner, Mirjam , Gonnella, G., Kurtz, S. and LaRoche, Julie (2014) Handling Temperature Bursts Reaching 464 C: Different Microbial Strategies in the Sisters Peak Hydrothermal Chimney. Applied and Environmental Microbiology, 80 (15). pp. 4585-4598. DOI 10.1128/AEM.01460-14.

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Abstract

The active venting Sisters Peak (SP) chimney on the Mid-Atlantic Ridge holds the current temperature record for the hottest ever measured hydrothermal fluids (400 degrees C, accompanied by sudden temperature bursts reaching 464 degrees C). Given the unprecedented temperature regime, we investigated the biome of this chimney with a focus on special microbial adaptations for thermal tolerance. The SP metagenome reveals considerable differences in the taxonomic composition from those of other hydrothermal vent and subsurface samples; these could be better explained by temperature than by other available abiotic parameters. The most common species to which SP genes were assigned were thermophilic Aciduliprofundum sp. strain MAR08-339 (11.8%), Hippea maritima (3.8%), Caldisericum exile (1.5%), and Caminibacter mediatlanticus (1.4%) as well as to the mesophilic Niastella koreensis (2.8%). A statistical analysis of associations between taxonomic and functional gene assignments revealed specific overrepresented functional categories: for Aciduliprofundum, protein biosynthesis, nucleotide metabolism, and energy metabolism genes; for Hippea and Caminibacter, cell motility and/or DNA replication and repair system genes; and for Niastella, cell wall and membrane biogenesis genes. Cultured representatives of these organisms inhabit different thermal niches; i.e., Aciduliprofundum has an optimal growth temperature of 70 degrees C, Hippea and Caminibacter have optimal growth temperatures around 55 degrees C, and Niastella grows between 10 and 37 degrees C. Therefore, we posit that the different enrichment profiles of functional categories reflect distinct microbial strategies to deal with the different impacts of the local sudden temperature bursts in disparate regions of the chimney.

Document Type: Article
Additional Information: WOS:000338707800013; Supplemental Material: Overview of the upstream (A) and downstream (B) analysis pipeline (Fig. S1); collectors' curves and Shannon diversity indices for the tested metagenomes from Sisters Peak, Juan de Fuca, Mariana Trough, and Brazos Trinity Basin based on taxonomic assignments at species, genus, family, order, class, and phylum levels (Fig. S2); analysis of the variability of SP metagenome sequences recruited to the reference genomes of Aciduliprofundum sp. MAR08-339 and Aciduliprofundum boonei T469 (Fig. S3); recruitment plots of SP metagenome fragments to the reference genomes of Aciduliprofundum sp. MAR08-339, Aciduliprofundum boonei T469, Hippea maritime DSM10411, Caldisericum exile AZM16c01, and Niastella koreensis GM20-10 (Fig. S4); metadata of sequenced metagenomes used for comparison (Table S1); summary of the taxonomic and functional assignment results (Table S2); taxonomic assignment of predicted rRNA genes (Table S3); potential genomic islands of Aciduliprofundum sp. MAR08-339, respective matches in the SP metagenome, and other best hits (Table S4).
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1128/AEM.01460-14
ISSN: 0099-2240
Projects: Future Ocean
Date Deposited: 19 Aug 2014 08:26
Last Modified: 18 Jul 2018 08:07
URI: http://oceanrep.geomar.de/id/eprint/25466

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