A Fast and Validated Method for the Determination of Malondialdehyde in Fish Liver Using High-Performance Liquid Chromatography with a Photodiode Array Detector.

Faizan, Mohammad, Esatbeyoglu, Tuba, Bayram, Banu and Rimbach, Gerald (2014) A Fast and Validated Method for the Determination of Malondialdehyde in Fish Liver Using High-Performance Liquid Chromatography with a Photodiode Array Detector. Journal of Food Science, 79 (4). C484-C488. DOI 10.1111/1750-3841.12412.

Full text not available from this repository.

Supplementary data:

Abstract

Malondialdehyde (MDA) is a biomarker of lipid peroxidation and is present in foods and biological samples such as plasma. A high-performance liquid chromatography (HPLC) method was applied to determine MDA in fish liver samples after derivatization with 2,4-dinitrophenylhydrazine (DNPH) using a ODS2 column (10 cm x 4.6 mm, 3 m) and a photodiode array detector. The mobile phase consisted of 0.2% acetic acid (v/v) in distilled water and acetonitrile (42:58, v/v). The present method was validated in terms of linearity, lower limit of quantification, lower limit of detection, precision, accuracy, recovery, and stability of MDA according to U.S. Food and Drug Administration (FDA) guidelines. The limit of quantification of MDA was 0.39 mol/L, which is comparable to other methods. The recovery of the spiked MDA liver samples was in the range of 92.4% to 104.2%. This newly modified HPLC method is specific, sensitive, and accurate and allows the analysis of MDA within 4 min in fish liver but also in other tissues and plasma. Practical Application Malondialdehyde is an established biomarker of lipid peroxidation in foods. We developed a fast, simple, and sensitive HPLC method with photodiode array detection using DNPH for derivatization of malondialdehyde in fish liver.

Document Type: Article
Additional Information: Times Cited: 0 0
Research affiliation: Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1111/1750-3841.12412
ISSN: 0022-1147
Projects: Future Ocean
Date Deposited: 30 Mar 2015 12:11
Last Modified: 23 Sep 2019 22:19
URI: http://oceanrep.geomar.de/id/eprint/27492

Actions (login required)

View Item View Item