Comparative QTL analysis of root lesion nematode resistance in barley.

Galal, Ahmed, Sharma, Shiveta, Abou-Elwafa, Salah Fatouh, Sharma, Shailendra, Kopisch-Obuch, Friedrich, Laubach, Eberhard, Perovic, Dragan, Ordon, Frank and Jung, Christian (2014) Comparative QTL analysis of root lesion nematode resistance in barley. Theoretical and Applied Genetics, 127 (6). pp. 1399-1407. DOI 10.1007/s00122-014-2307-x.

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Abstract

This study demonstrates for the first time that resistance to different root lesion nematodes ( P. neglectus and P. penetrans ) is controlled by a common QTL. A major resistance QTL ( Rlnnp6H ) has been mapped to chromosome 6H using two independent barley populations. Root lesion nematodes (Pratylenchus spp.) are important pests in cereal production worldwide. We selected two doubled haploid populations of barley (Igri x Franka and Uschi x HHOR 3073) and infected them with Pratylenchus penetrans and Pratylenchus neglectus. Nematode multiplication rates were measured 7 or 10 weeks after infection. In both populations, continuous phenotypic variations for nematode multiplication rates were detected indicating a quantitative inheritance of resistance. In the Igri x Franka population, four P. penetrans resistance QTLs were mapped with 857 molecular markers on four linkage groups (2H, 5H, 6H and 7H). In the Uschi x HHOR 3073 population, eleven resistance QTLs (P. penetrans and P. neglectus) were mapped with 646 molecular markers on linkage groups 1H, 3H, 4H, 5H, 6H and 7H. A major resistance QTL named Rlnnp6H (LOD score 6.42-11.19) with a large phenotypic effect (27.5-36.6 %) for both pests was mapped in both populations to chromosome 6H. Another resistance QTL for both pests was mapped on linkage group 5H (Igri x Franka population). These data provide first evidence for common resistance mechanisms against different root lesion nematode species. The molecular markers are a powerful tool for the selection of resistant barley lines among segregating populations because resistance tests are time consuming and laborious.

Document Type: Article
Additional Information: Times Cited: 0 Perovic, Dragan/C-1149-2008 Perovic, Dragan/0000-0002-0292-1693 0
Keywords: Double Haploid Double Haploid Line Linkage Group Double Haploid Population Resistance QTLs
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1007/s00122-014-2307-x
ISSN: 0040-5752
Projects: Future Ocean
Date Deposited: 30 Mar 2015 12:14
Last Modified: 27 Jun 2019 08:44
URI: http://oceanrep.geomar.de/id/eprint/27537

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