Effects of beta-Lactam Antibiotics and Fluoroquinolones on Human Gut Microbiota in Relation to Clostridium difficile Associated Diarrhea.

Knecht, Henrik, Neulinger, Sven C., Heinsen, Femke Anouska, Knecht, Carolin, Schilhabel, Anke, Schmitz, Ruth A., Zimmermann, Alexandra, dos Santos, Vitor Martins, Ferrer, Manuel, Rosenstiel, Philip, Schreiber, Stefan, Friedrichs, Anette K. and Ott, Stephan (2014) Effects of beta-Lactam Antibiotics and Fluoroquinolones on Human Gut Microbiota in Relation to Clostridium difficile Associated Diarrhea. Open Access PLoS ONE, 9 (2). DOI 10.1371/journal.pone.0089417.

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Supplementary data:


Clostridium difficile infections are an emerging health problem in the modern hospital environment. Severe alterations of the gut microbiome with loss of resistance to colonization against C. difficile are thought to be the major trigger, but there is no clear concept of how C. difficile infection evolves and which microbiological factors are involved. We sequenced 16S rRNA amplicons generated from DNA and RNA/cDNA of fecal samples from three groups of individuals by FLX technology: (i) healthy controls (no antibiotic therapy); (ii) individuals receiving antibiotic therapy (Ampicillin/Sulbactam, cephalosporins, and fluoroquinolones with subsequent development of C. difficile infection or (iii) individuals receiving antibiotic therapy without C. difficile infection. We compared the effects of the three different antibiotic classes on the intestinal microbiome and the effects of alterations of the gut microbiome on C. difficile infection at the DNA (total microbiota) and rRNA (potentially active) levels. A comparison of antibiotic classes showed significant differences at DNA level, but not at RNA level. Among individuals that developed or did not develop a C. difficile infection under antibiotics we found no significant differences. We identified single species that were up-or down regulated in individuals receiving antibiotics who developed the infection compared to non-infected individuals. We found no significant differences in the global composition of the transcriptionally active gut microbiome associated with C. difficile infections. We suggest that up-and down regulation of specific bacterial species may be involved in colonization resistance against C. difficile providing a potential therapeutic approach through specific manipulation of the intestinal microbiome.

Document Type: Article
Additional Information: Times Cited: 0 0
Keywords: Clostridium difficile Antibiotics Gut bacteria Microbiome Gastrointestinal tract Bacteria Diarrhea Ribosomal RNA
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1371/journal.pone.0089417
ISSN: 1932-6203
Projects: Future Ocean
Date Deposited: 30 Mar 2015 12:28
Last Modified: 29 Jun 2019 09:35
URI: http://oceanrep.geomar.de/id/eprint/27773

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