Toxicity of Functional Nano-Micro Zinc Oxide Tetrapods: Impact of Cell Culture Conditions, Cellular Age and Material Properties.

Papavlassopoulos, Heike, Mishra, Yogendra K., Kaps, Soeren, Paulowicz, Ingo, Abdelaziz, Ramzy, Elbahri, Mady, Maser, Edmund, Adelung, Rainer, Roehl, Claudia and Wei-Chun, Chin (2014) Toxicity of Functional Nano-Micro Zinc Oxide Tetrapods: Impact of Cell Culture Conditions, Cellular Age and Material Properties. Open Access PLoS ONE, 9 (1). DOI 10.1371/journal.pone.0084983.

Full text not available from this repository.

Supplementary data:

Abstract

With increasing production and applications of nanostructured zinc oxide, e. g., for biomedical and consumer products, the question of safety is getting more and more important. Different morphologies of zinc oxide structures have been synthesized and accordingly investigated. In this study, we have particularly focused on nano-micro ZnO tetrapods (ZnO-T), because their large scale fabrication has been made possible by a newly introduced flame transport synthesis approach which will probably lead to several new applications. Moreover, ZnO-T provide a completely different morphology then classical spherical ZnO nanoparticles. To get a better understanding of parameters that affect the interactions between ZnO-T and mammalian cells, and thus their biocompatibility, we have examined the impact of cell culture conditions as well as of material properties on cytotoxicity. Our results demonstrate that the cell density of fibroblasts in culture along with their age, i.e., the number of preceding cell divisions, strongly affect the cytotoxic potency of ZnO-T. Concerning the material properties, the toxic potency of ZnO-T is found to be significantly lower than that of spherical ZnO nanoparticles. Furthermore, the morphology of the ZnO-T influenced cellular toxicity in contrast to surface charges modified by UV illumination or O-2 treatment and to the material age. Finally, we have observed that direct contact between tetrapods and cells increases their toxicity compared to transwell culture models which allow only an indirect effect via released zinc ions. The results reveal several parameters that can be of importance for the assessment of ZnO-T toxicity in cell cultures and for particle development.

Document Type: Article
Additional Information: Times Cited: 9 Mishra, Yogendra/G-6241-2010 Mishra, Yogendra/0000-0002-8786-9379 0 9
Keywords: Cytotoxicity Toxicity Fibroblasts Cell cultures Zinc Oxygen Scanning electron microscopy Nanomaterials
Research affiliation: Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.1371/journal.pone.0084983
ISSN: 1932-6203
Projects: Future Ocean
Date Deposited: 30 Mar 2015 12:36
Last Modified: 23 Sep 2019 22:16
URI: http://oceanrep.geomar.de/id/eprint/28094

Actions (login required)

View Item View Item