Chemical characterization and quantification of the brown algal storage compound laminarin — A new methodological approach.

Graiff, Angelika, Ruth, Wolfgang, Kragl, Udo and Karsten, Ulf (2016) Chemical characterization and quantification of the brown algal storage compound laminarin — A new methodological approach. Journal of Applied Phycology, 28 (1). pp. 533-543. DOI 10.1007/s10811-015-0563-z.

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Abstract

The polysaccharide laminarin (β-1,3-glucan) is used as a long-term carbon storage compound in brown algae. This chemical storage form of carbon enables perennial brown algae in seasonally fluctuating ecosystems to uncouple growth from photosynthesis, i.e., most of these plants grow as seasonal anticipators in winter based on remobilization of laminarin, while in summer, growth typically ceased to fill up the storage pool. Because of this high ecological relevance, a reliable and precise method for determination and quantification of laminarin is needed. Therefore, a simple, efficient, cold water extraction method coupled to a new quantitative liquid chromatography-mass spectrometrical method (LC-MS) was developed. Laminarin was determined in 9 out of 12 brown algal species, and its expected typical molar mass distribution of 2000–7000 Da was confirmed. Furthermore, laminarin consisted of a complex mixture of different chemical forms, since 15 chemical laminarin species with distinct molecular weights were measured in 9 species of brown algae. Differences in chain length and number of laminarin species seem to be species specific and hence may indicate some chemotaxonomic value. Laminarin concentrations in the algal tissues ranged from 0.03 to 0.86 % dry weight (DW). The direct chemical characterization and quantification of laminarin by LC-MS represents a powerful method to verify the biochemical and ecological importance of laminarin for brown algae.

Document Type: Article
Keywords: Kelps; LC-MS; Mannitol; Seasonal growth; Carbon storage
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1007/s10811-015-0563-z
ISSN: 0921-8971
Projects: BIOACID
Date Deposited: 27 Apr 2015 12:40
Last Modified: 06 Aug 2019 13:14
URI: http://oceanrep.geomar.de/id/eprint/28674

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