Assessing the potential of amino acid 13 patterns as a carbon source tracer in marine sediments: effects of algal growth conditions and sedimentary diagenesis

Larsen, Thomas, Bach, Lennart T., Salvatteci, Renato, Wang, Y. V., Andersen, N., Ventura, M. and McCarthy, M. D. (2015) Assessing the potential of amino acid 13 patterns as a carbon source tracer in marine sediments: effects of algal growth conditions and sedimentary diagenesis Biogeosciences (BG), 12 (16). pp. 4979-4992. DOI 10.5194/bg-12-4979-2015.

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Abstract

Burial of organic carbon in marine sediments has a profound influence in marine biogeochemical cycles and provides a sink for greenhouse gases such as CO2 and CH4. However, tracing organic carbon from primary production sources as well as its transformations in the sediment record remains challenging. Here we examine a novel but growing tool for tracing the biosynthetic origin of amino acid carbon skeletons, based on naturally occurring stable carbon isotope patterns in individual amino acids (δ13CAA). We focus on two important aspects for δ13CAA utility in sedimentary paleoarchives: first, the fidelity of source diagnostic of algal δ13CAA patterns across different oceanographic growth conditions, and second, the ability of δ13CAA patterns to record the degree of subsequent microbial amino acid synthesis after sedimentary burial. Using the marine diatom Thalassiosira weissflogii, we tested under controlled conditions how δ13CAA patterns respond to changing environmental conditions, including light, salinity, temperature, and pH. Our findings show that while differing oceanic growth conditions can change macromolecular cellular composition, δ13CAA isotopic patterns remain largely invariant. These results emphasize that δ13CAA patterns should accurately record biosynthetic sources across widely disparate oceanographic conditions. We also explored how δ13CAA patterns change as a function of age, total nitrogen and organic carbon content after burial, in a marine sediment core from a coastal upwelling area off Peru. Based on the four most informative amino acids for distinguishing between diatom and bacterial sources (i.e., isoleucine, lysine, leucine and tyrosine), bacterially derived amino acids ranged from 10 to 15 % in the sediment layers from the last 5000 years, and up to 35 % during the last glacial period. The greater bacterial contributions in older sediments indicate that bacterial activity and amino acid resynthesis progressed, approximately as a function of sediment age, to a substantially larger degree than suggested by changes in total organic nitrogen and carbon content. It is uncertain whether archaea may have contributed to sedimentary δ13CAA patterns we observe, and controlled culturing studies will be needed to investigate whether δ13CAA patterns can differentiate bacterial from archeal sources. Further research efforts are also needed to understand how closely δ13CAA patterns derived from hydrolyzable amino acids represent total sedimentary proteineincous material, and more broadly sedimentary organic nitrogen. Overall, however, both our culturing and sediment studies suggest that δ13CAA patterns in sediments will represent a novel proxy for understanding both primary production sources, and the direct bacterial role in the ultimate preservation of sedimentary organic matter.

Document Type: Article
Additional Information: WOS:000360294800008
Keywords: ISOTOPIC FRACTIONATION PATTERNS; CONTINENTAL-MARGIN SEDIMENTS; DISSOLVED ORGANIC-MATTER; RATIO MASS-SPECTROMETRY; BIOCHEMICAL-COMPOSITION; BACTERIAL CONTRIBUTION; NITROGEN; PRESERVATION; OCEAN; PARTICULATE
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence > FO-R03
OceanRep > SFB 754 > A6
Kiel University > Faculty of Mathematics and Natural Sciences > Institute of Geosciences
OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > SFB 754
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Kiel University > Other > Leibniz-Laboratory for Radiometric Dating and Isotope Research
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.5194/bg-12-4979-2015
ISSN: 1726-4170
Projects: BIOACID, Future Ocean, SFB754
Date Deposited: 25 Aug 2015 10:13
Last Modified: 05 Apr 2017 11:54
URI: http://oceanrep.geomar.de/id/eprint/29408

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