Development and characterization of microsatellite loci for the haploid–diploid red seaweed Gracilaria vermiculophylla.

Kollars, Nicole M., Krueger-Hadfield, Stacy A., Byers, James E., Greig, Thomas W., Strand, Allan E., Weinberger, Florian and Sotka, Erik E. (2015) Development and characterization of microsatellite loci for the haploid–diploid red seaweed Gracilaria vermiculophylla. Open Access PeerJ, 3 . e1159. DOI 10.7717/peerj.1159.

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Abstract

Microsatellite loci are popular molecular markers due to their resolution in distinguishing individual genotypes. However, they have rarely been used to explore the population dynamics in species with biphasic life cycles in which both haploid and diploid stages develop into independent, functional organisms. We developed microsatellite loci for the haploid–diploid red seaweed Gracilaria vermiculophylla, a widespread non-native species in coastal estuaries of the Northern hemisphere. Forty-two loci were screened for amplification and polymorphism. Nine of these loci were polymorphic across four populations of the extant range with two to eleven alleles observed. Mean observed and expected heterozygosities ranged from 0.265 to 0.527 and 0.317 to 0.387, respectively. Overall, these markers will aid in the study of the invasive history of this seaweed and further studies on the population dynamics of this important haploid–diploid primary producer.

Document Type: Article
Additional Information: Data links: Table 2: Characteristics of nine polymorphic microsatellite loci developed for Gracilaria vermiculophylla. Acc. No., GenBank accession number; locus; motif; primer sequences; allele range; avg. error: TANDEM (Matschiner & Saltzburger, 2009) rounding errors for each microsatellite locus (the authors of TANDEM suggest that good loci have an average rounding error which is below 10% of the repeat size); Ntall, total number of alleles. All loci showed one-locus genetic determinism. WOS:000360845100004
Keywords: Complex life cycles, Biological invasions, Seaweed, Microsatellites, Haploid-diploid, Gracilaria vermiculophylla
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.7717/peerj.1159
ISSN: 2167-8359
Related URLs:
Projects: Future Ocean
Date Deposited: 07 Sep 2015 09:44
Last Modified: 23 Jan 2019 12:30
URI: http://oceanrep.geomar.de/id/eprint/29523

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