Separation and Detection of Siderophores Produced by Marine Bacterioplankton Using High-Performance Liquid Chromatography with Electrospray Ionization Mass Spectrometry.

McCormack, Paul, Worsfold, Paul J. and Gledhill, Martha (2003) Separation and Detection of Siderophores Produced by Marine Bacterioplankton Using High-Performance Liquid Chromatography with Electrospray Ionization Mass Spectrometry. Analytical Chemistry, 75 (11). pp. 2647-2652. DOI 10.1021/ac0340105.

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Abstract

High-performance liquid chromatography-electrospray ionization mass spectrometry was applied to the detection of the iron(III) complexes of the hydroxamate siderophores rhodotoluric acid, deferrioxamine B, and deferrichrome. Separation of the iron(III) complexes was obtained using a polystyrene-divinylbenzene stationary phase. The retention and responses of ferrioxamine and ferrichrome were optimal when a gradient elution program with methanol and 0.1% (v/v) formic acid as the mobile phases was used. These conditions were also suitable for the retention and separation of the uncomplexed ligands. Retention of iron(III) rhodotoluate was improved when formic acid was replaced by the ion-pairing reagent heptafluorobutyric acid (0.1%). Detection limits for the ferric complexes, defined as 3 SD of the lowest determined standard, were 26 nM for iron(III) rhodotoluate, 0.23 nM for ferrioxamine, and 0.40 nM for ferrichrome. A protocol for the solid-phase extraction of these hydroxamate siderophores from seawater was developed and applied to the extraction of siderophores from enriched incubated seawater samples.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-CH Chemical Oceanography
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1021/ac0340105
ISSN: 0003-2700
Date Deposited: 15 Sep 2015 14:08
Last Modified: 30 May 2017 09:18
URI: http://oceanrep.geomar.de/id/eprint/29692

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