eDNA and specific primers for early detection of invasive species – A case study on the bivalve Rangia cuneata, currently spreading in Europe.

Ardura, Alba, Zaiko, Anastasija, Martinez, Jose L., Samulioviene, Aurelija, Semenova, Anna and Garcia-Vazquez, Eva (2015) eDNA and specific primers for early detection of invasive species – A case study on the bivalve Rangia cuneata, currently spreading in Europe. Marine Environmental Research, 112(B) . pp. 48-55. DOI 10.1016/j.marenvres.2015.09.013.

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Abstract

Intense human activities facilitate the successful spread and establishment of non-indigenous aquatic organisms in marine and freshwater ecosystems. In some cases such intrusions result in noticeable and adverse changes in the recipient environments. In the Baltic Sea, the discovery and rapid initial spread of the North American wedge clam Rangia cuneata represents a new wave of invasion which may trigger unpredictable changes of the local benthic communities. In this study we present a species-specific DNA-based marker developed in silico and experimentally tested on environmental samples. Marker specificity and sensitivity were assessed in vitro from water samples containing different mixtures of the target species and other five bivalves currently present in the region: the native Cerastoderma glaucum, Macoma balthica and Mytilus trossulus, the invasive Dreissena polymorpha and the cryptogenic Mya arenaria. Cross-species amplification was not found in any case. The method allows to detecting at least 0.4 ng of Rangia cuneata DNA per μl, and 0.1 g of tissue per liter of water. Finally, the marker performance was assessed in water samples from the Baltic Sea and Vistula Lagoon. The coincidence between independent visual observations of Rangia cuneata and positive PCR amplification of the marker from the water samples confirmed the efficiency of this highly reproducible, fast, and technically easy method. Rangia cuneata traces can be detected from environmental DNA even when the population is sparse and small, enabling rapid management responses and allowing to track the invasion dynamics.

Document Type: Article
Keywords: Baltic Sea; Non-indigenous species; Species-specific marker; Rangia cuneata; eDNA; Vistula Lagoon
Refereed: Yes
DOI etc.: 10.1016/j.marenvres.2015.09.013
ISSN: 0141-1136
Related URLs:
Projects: BONUS BIO-C3
Date Deposited: 02 Dec 2015 09:11
Last Modified: 02 Dec 2015 09:11
URI: http://oceanrep.geomar.de/id/eprint/30409

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