Acid–base balance and changes in haemolymph properties of the South African rock lobsters, Jasus lalandii, a palinurid decapod, during chronic hypercapnia

Knapp, Jarred L., Bridges, Christopher R., Krohn, Janina, Hoffman, Louwrens C. and Auerswald, Lutz (2015) Acid–base balance and changes in haemolymph properties of the South African rock lobsters, Jasus lalandii, a palinurid decapod, during chronic hypercapnia Biochemical and Biophysical Research Communications, 461 (3). pp. 475-480. DOI 10.1016/j.bbrc.2015.04.025.

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Abstract

Few studies exist reporting on long-term exposure of crustaceans to hypercapnia. We exposed juvenile South African rock lobsters, Jasus lalandii, to hypercapnic conditions of pH 7.3 for 28 weeks and subsequently analysed changes in the extracellular fluid (haemolymph). Results revealed, for the first time, adjustments in the haemolymph of a palinurid crustacean during chronic hypercapnic exposure: 1) acid-base balance was adjusted and sustained by increased bicarbonate and 2) quantity and oxygen binding properties of haemocyanin changed. Compared with lobsters kept under normocapnic conditions (pH 8.0), during prolonged hypercapnia, juvenile lobsters increased bicarbonate buffering of haemolymph. This is necessary to provide optimum pH conditions for oxygen binding of haemocyanin and functioning of respiration in the presence of a strong Bohr Effect. Furthermore, modification of the intrinsic structure of the haemocyanin molecule, and not the presence of molecular modulators, seems to improve oxygen affinity under conditions of elevated pCO2.

Document Type: Article
Keywords: Acid-base balance; Chronic hypercapnia; Haemoprotein haemocyanin; Molecular structure change; Ocean acidification
Refereed: Yes
DOI etc.: 10.1016/j.bbrc.2015.04.025
ISSN: 0006-291X
Projects: BIOACID
Date Deposited: 12 Feb 2016 12:31
Last Modified: 12 Feb 2016 12:31
URI: http://oceanrep.geomar.de/id/eprint/31333

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