Identification of the Scopularide Biosynthetic Gene Cluster in Scopulariopsis brevicaulis.

Lukassen, Mie Bech, Saei, Wagma, Sondergaard, Teis Esben, Tamminen, Anu, Kumar, Abhishek, Kempken, Frank, Wiebe, Marilyn G. and Sorensen, Jens Laurids (2015) Identification of the Scopularide Biosynthetic Gene Cluster in Scopulariopsis brevicaulis. Open Access Marine Drugs, 13 (7). pp. 4331-4343. DOI 10.3390/md13074331.

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Abstract

Scopularide A is a promising potent anticancer lipopeptide isolated from a marine derived Scopulariopsis brevicaulis strain. The compound consists of a reduced carbon chain (3-hydroxy-methyldecanoyl) attached to five amino acids (glycine, l-valine, d-leucine, l-alanine, and l-phenylalanine). Using the newly sequenced S. brevicaulis genome we were able to identify the putative biosynthetic gene cluster using genetic information from the structurally related emericellamide A from Aspergillus nidulans and W493-B from Fusarium pseudograminearum. The scopularide A gene cluster includes a nonribosomal peptide synthetase (NRPS1), a polyketide synthase (PKS2), a CoA ligase, an acyltransferase, and a transcription factor. Homologous recombination was low in S. brevicaulis so the local transcription factor was integrated randomly under a constitutive promoter, which led to a three to four-fold increase in scopularide A production. This indirectly verifies the identity of the proposed biosynthetic gene cluster.

Document Type: Article
Additional Information: Times Cited: 2
Keywords: non-ribosomal peptide synthetases; polyketide synthases; secondary metabolites; transcription factor; Fusarium; regulation; marine fungi
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence > FO-R03
Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.3390/md13074331
ISSN: 1660-3397
Projects: Future Ocean
Date Deposited: 18 Oct 2016 03:46
Last Modified: 23 Sep 2019 20:43
URI: http://oceanrep.geomar.de/id/eprint/32576

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