Phylogenetic diversity and antimicrobial activities of bryozoan-associated bacteria isolated from Mediterranean and Baltic Sea habitats.

Heindl, Herwig, Wiese, Jutta , Thiel, Vera and Imhoff, Johannes F. (2010) Phylogenetic diversity and antimicrobial activities of bryozoan-associated bacteria isolated from Mediterranean and Baltic Sea habitats. Systematic and Applied Microbiology, 33 . pp. 94-104. DOI 10.1016/j.syapm.2009.12.002.

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Abstract

To date, only a small number of investigations covering microbe–bryozoa associations have been carried out. Most of them have focused on a few bryozoan species and none have covered the antibacterial activities of associated bacteria. In the current study, the proportion and phylogenetic classification of Bryozoan-associated bacteria with antimicrobial properties were investigated. Twenty-one specimens of 14 different bryozoan species were collected from several sites in the Baltic and the Mediterranean Sea. A total of 340 associated bacteria were isolated, and 101 displayed antibiotic activities. While antibiosis was predominantly directed against Gram-positive test strains, 16S rRNA gene sequencing revealed affiliation of the isolates to Gram-negative classes (Flavobacteria, Alpha- and Gammaproteobacteria). One isolate was related to the Gram-positive Actinobacteria. The sequences were grouped into 27 phylotypes on the basis of similarity values ≥99.5%. A host-specific affiliation was not revealed as members of the same phylotype were derived from different bryozoan species. Site-specific patterns, however, were demonstrated. Strains of the genera Sphingomonas and Alteromonas were exclusively isolated from Mediterranean sites, whereas Shewanella, Marinomonas and Vibrio-related isolates were only from Baltic sites. Although Pseudoalteromonas affiliated strains were found in both habitats, they were separated into respective phylotypes. Isolates with 16S rDNA similarity values <98%, which could possibly represent new species, belonged to the genera Shewanella, Pseudoalteromonas and Tenacibaculum.

Document Type: Article
Additional Information: WOS:000277032000007
Keywords: Bryozoa, Antimicrobial activity, Baltic Sea, Mediterranean Sea, Host-bacteria association, 16S rRNA gene analysis; CANDIDATUS-ENDOBUGULA-SERTULA; SPONGE-ASSOCIATED BACTERIUM; MARINE NATURAL-PRODUCTS; BUGULA-NERITINA; SP-NOV.; PALEOECOLOGICAL IMPLICATIONS; PSYCHROTROPHIC BACTERIA; SUBTROPICAL LATITUDES; SECONDARY METABOLITES; MICROBIAL COMMUNITIES
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > GEOMAR > Applied R&D > Centre for Marine Substances (KiWiZ)
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.syapm.2009.12.002
ISSN: 0723-2020
Related URLs:
Projects: Future Ocean
Date Deposited: 24 Mar 2010 09:13
Last Modified: 17 Jun 2016 09:40
URI: http://oceanrep.geomar.de/id/eprint/3273

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