Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 — studies in cultured HT-29 cells and mice.

Wagner, Anika E., Sturm, Christine, Piegholdt, Stefanie, Wolf, Insa M. A., Esatbeyoglu, Tuba, De Nicola, Gina Rosalinda, Iori, Renato and Rimbach, Gerald (2015) Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 — studies in cultured HT-29 cells and mice. The Journal of Nutritional Biochemistry, 26 (6). pp. 661-666. DOI 10.1016/j.jnutbio.2015.01.004.

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Abstract

In this study, the effect of myrosinase-treated glucoerucin (GER+MYR), which releases the isothiocyanate (ITC) erucin, on heme oxygenase 1 (HO-1) gene expression and Nrf2 signaling was investigated in vitro in cultured cells and in vivo in mice. Treatment of HT-29 cells with GER+MYR resulted in a significant increase in the mRNA and protein levels of nuclear Nrf2 and HO-1. GER+MYR was more potent at enhancing the nuclear Nrf2 levels than were the following myrosinase-treated glucosinolates: sinigrin, glucoraphanin and gluconasturtiin, which are the precursors of allyl-ITC, R-sulforaphane and 2-phenylethyl ITC, respectively. GER+MYR also significantly induced HO-1 gene expression in the mouse intestinal mucosae and liver but not in the brain. Mechanistic studies suggest that GER+MYR induces Nrf2 via ERK1/2-, p38- and JNK-dependent signal transduction pathways. The GER+MYR-mediated increase in HO-1 expression is primarily attributable to p38 signaling. (C) 2015 Elsevier Inc. All rights reserved.

Document Type: Article
Additional Information: Times Cited: 0
Keywords: Glucoerucin, Heme oxygenase 1, Nrf2, Glucosinolates, Myrosinase, MAPK
Research affiliation: Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
Publisher: Elsevier
Projects: Future Ocean
Date Deposited: 18 Oct 2016 03:46
Last Modified: 23 Sep 2019 20:56
URI: https://oceanrep.geomar.de/id/eprint/32742

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