Phylogenetic Relationship and Secondary Metabolite Production of Marine Fungi Producing the Cyclodepsipeptides Scopularide A and B.

Kramer, Annemarie, Labes, Antje and Imhoff, Johannes F. (2016) Phylogenetic Relationship and Secondary Metabolite Production of Marine Fungi Producing the Cyclodepsipeptides Scopularide A and B. Marine Biotechnology, 18 (4). pp. 466-474. DOI 10.1007/s10126-016-9707-7.

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Abstract

Strains originally affiliated to the genera Scopulariopsis and Microascus were compared regarding the scopularide production in order to investigate their ability to produce the cyclodepsipeptides and select the best suited candidate for subsequent optimisation processes. Phylogenetic calculations using available sequences of the genera Scopulariopsis and Microascus revealed that most of the sequences clustered within two closely related groups, comprising mainly Scopulariopsis/Microascus brevicaulis and Microascus sp., respectively. Interestingly, high yields of scopularide A were exhibited by three strains belonging to S./M. brevicaulis, while lower titres were observed for two strains of Microascus sp. Close phylogenetic distances within and between the two groups supported the proposed combination of both genera into one holomorph group. Short phylogenetic distances did not allow a clear affiliation at the species level on the basis of ribosomal DNA sequences, especially for Microascus sp. strains. Additionally, several sequences originating from strains assigned to Scopulariopsis exhibited a polyphyletic nature. The production pattern is in accordance with the phylogenetic position of the strains and significant production of scopularide B could only be observed for the S./M. brevicaulis strain LF580. Thus, the phylogenetic position marks the biotechnologically interesting strains and matters in optimisation strategies. In conclusion, the ability of all five strains to produce at least one of the scopularides suggests a distribution of the responsible gene cluster within the holomorph group. Setting the focus on the production of the cyclodepsipeptides, strain LF580 represents the best candidate for further strain and process optimisation

Document Type: Article
Funder compliance: info:eu-repo/grantAgreement/EC/FP7/265926
Keywords: Scopulariopsis/Microascus brevicaulis LF580; Microascus sp.; Phylogeny of secondary metabolite producers; Natural products; Anamorph-teleomorph
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1007/s10126-016-9707-7
ISSN: 1436-2228
Related URLs:
Projects: Marine Fungi
Date Deposited: 16 Jun 2016 10:25
Last Modified: 01 Feb 2019 15:11
URI: http://oceanrep.geomar.de/id/eprint/33138

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