The Bunsen gas solubility coefficient of ethylene as a function of temperature and salinity and its importance for nitrogen fixation assays.

Breitbarth, Eike, Mills, Matthew M., Friedrichs, Gernot and LaRoche, Julie (2004) The Bunsen gas solubility coefficient of ethylene as a function of temperature and salinity and its importance for nitrogen fixation assays. Limnology and Oceanography: Methods, 2 . pp. 282-288. DOI 10.4319/lom.2004.2.282.

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Abstract

The acetylene reduction assay is a common method for assessing nitrogen fixation in a variety of marine and freshwater systems. The method measures ethylene, the product of the conversion of the gas acetylene to its reduced form by nitrogenase. Knowledge of the solubility of ethylene in aqueous solution is crucial to the calculation of nitrogen fixation rates and depends on the temperature and salinity of the assay conditions. Despite the increasing interest in marine nitrogen fixation, no gas solubility (Bunsen) coefficients for ethylene in seawater are published to date. Here, we provide a set of equations and present semiempirically derived Bunsen coefficients for ethylene in water (ranging from 0.069 to 0.226) for a range of temperatures and salinities that are relevant for aquatic nitrogen fixation. We apply these data to nitrogen fixation scenarios at different temperatures and salinities and stress the importance of using accurate Bunsen coefficients in nitrogen fixation assays.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.4319/lom.2004.2.282
ISSN: 1541-5856
Projects: IRONAGES
Date Deposited: 07 Dec 2009 13:50
Last Modified: 14 Aug 2018 12:35
URI: http://oceanrep.geomar.de/id/eprint/3320

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