A shell regeneration assay to identify biomineralization candidate genes in mytilid mussels

Hüning, Anne K., Lange, Skadi, Ramesh, Kirti, Jacob, Dorrit E., Jackson, Daniel J., Panknin, Ulrike, Gutowska, Magdalena A., Philipp, Eva E. R., Rosenstiel, Philip, Lucassen, Magnus and Melzner, Frank (2016) A shell regeneration assay to identify biomineralization candidate genes in mytilid mussels Marine Genomics, 27 . pp. 57-67. DOI 10.1016/j.margen.2016.03.011.

[img] Text
Hüning.pdf - Published Version
Restricted to Registered users only

Download (1414Kb) | Contact

Supplementary data:

Abstract

Biomineralization processes in bivalve molluscs are still poorly understood. Here we provide an analysis of specifically expressed sequences from a mantle transcriptome of the blue mussel, Mytilus edulis. We then developed a novel, integrative shell injury assay to test, whether biomineralization candidate genes highly expressed in marginal and pallial mantle could be induced in central mantle tissue underlying the damaged shell areas. This experimental approach makes it possible to identify gene products that control the chemical micro-environment during calcification as well as organic matrix components. This is unlike existing methodological approaches that work retroactively to characterize calcification relevant molecules and are just able to examine organic matrix components that are present in completed shells. In our assay an orthogonal array of nine 1 mm holes was drilled into the left valve, and mussels were suspended in net cages for 20, 29 and 36 days to regenerate. Structural observations using stereo-microscopy, SEM and Raman spectroscopy revealed organic sheet synthesis (day 20) as the first step of shell-repair followed by the deposition of calcite crystals (days 20 and 29) and aragonite tablets (day 36). The regeneration period was characterized by time-dependent shifts in gene expression in left central mantle tissue underlying the injured shell, (i) increased expression of two tyrosinase isoforms (TYR3: 29-fold and TYR6: 5-fold) at day 20 with a decline thereafter, (ii) an increase in expression of a gene encoding a nacrein-like protein (max. 100-fold) on day 29. The expression of an acidic Asp-Ser-rich protein was enhanced during the entire regeneration process. This proof-of-principle study demonstrates that genes that are specifically expressed in pallial and marginal mantle tissue can be induced (4 out of 10 genes) in central mantle following experimental injury of the overlying shell. Our findings suggest that regeneration assays can be used systematically to better characterize gene products that are essential for distinct phases of the shell formation process, particularly those that are not incorporated into the organic shell matrix.

Document Type: Article
Funder compliance: info:eu-repo/grantAgreement/EC/FP7/605051
Additional Information: WOS:000379376100008; PubMed ID: 27083865
Keywords: Biomineralization, Shell regeneration, Mytilus, Transcriptomics
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-MG Marine Geosystems
AWI
OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-BI Biological Oceanography
Kiel University
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.margen.2016.03.011
ISSN: 1874-7787
Related URLs:
Projects: BIOACID, CACHE
Date Deposited: 05 Aug 2016 09:25
Last Modified: 04 May 2017 11:08
URI: http://oceanrep.geomar.de/id/eprint/33531

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...