Divyasree, B., Ramana, Ch. V., Sasikala, Ch., Lakshmi, K. V. N. S. and Bharti, Dave (2016) Rhodovulum aestuarii sp. nov., isolated from a brackish water body. International Journal of Systematic and Evolutionary Microbiology, 66 (1). pp. 165-171. DOI 10.1099/ijsem.0.000691.

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Abstract

A yellowish brown, phototrophic, purple non-sulfur bacterium, strain JA924r, was isolated in pure culture from a brackish water sample collected from an estuary. Single cells were oval to rod-shaped, non-motile and Gram-stain-negative and had a vesicu!ar architecture of intracellular photosynthetic membranes. Bacteriochlorophyll-a and carotenoids of the spheroidene series were present as photosynthetic pigments. Photolithoautotrophy, chemo-organoheterotrophy and
photo-organoheterotrophy were the growth modes observed. Strain JA924T had complex
growth requ1rements. Strain JA924 T was mesophilic and moderate!y halophilic. The DNA G -t- C content was 64 mal% (HPLC). The major cellular fatty acids were C18 1f·)7c/C 18 : 11·)6c, Ct 6 0 and C 18 . 0 . The major quinone was ubiquinone-1 0 (0-1 0). Phosphatidylg!ycerol,
phosphatidylethanolamine, sulfolipid and an aminolipid were the main polar Iipids of strain JA924r. EzTaxon-e BLAST searches based on the 168 rRNA gene sequence of JA924T revealed highest similarity with Rhodovulum mangrovi AK41 T (98.19 %) and other members of the genus Rhodovulum (<95.71 %). Strain JA924T was further identified tobe distantly related to Rhodovulum mangrovi AK41 T ( <29% based on ONA-DNA hybridization and t1 Tm (>5 oc).
Phenotypic, chemotaxonomic and molecular differences indicate that strain JA924 T represents a novel species of the genus Rhodovulum, for which the name Rhodovulum aestuarii sp. nov. is proposed. The type strain is JA924 T ( = LMG 29031 T = KCTC 15485 T).

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