Evaluation of in vitro antiprotozoal activity of Ajuga laxmannii and its secondary metabolites.

Atay, Irem, Kirmizibekmez, Hasan, Kaiser, Marcel, Akaydin, Galip, Yesilada, Erdem and Tasdemir, Deniz (2016) Evaluation of in vitro antiprotozoal activity of Ajuga laxmannii and its secondary metabolites. Open Access Pharmaceutical Biology, 54 (9). pp. 1808-1814. DOI 10.3109/13880209.2015.1129542.

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Abstract

Abstract:
Context: Some Ajuga L. (Lamiaceae) species are traditionally used for the treatment of malaria, as well as fever, which is a common symptom of many parasitic diseases.

Objective: In the continuation of our studies on the identification of antiprotozoal secondary metabolites of Turkish Lamiaceae species, we have investigated the aerial parts of Ajuga laxmannii.

Materials and methods: The aerial parts of A. laxmannii were extracted with MeOH. The H2O subextract was subjected to polyamide, C18-MPLC and SiO2 CCs to yield eight metabolites. The structures of the isolates were elucidated by NMR spectroscopy and MS analyses. The extract, subextracts as well as the isolates were tested for their in vitro antiprotozoal activities against Plasmodium falciparum, Trypanasoma brucei rhodesiense, T. cruzi and Leishmania donovani at concentrations of 90–0.123 μg/mL.

Results: Two iridoid glycosides harpagide (1) and 8-O-acetylharpagide (2), three o-coumaric acid derivatives cis-melilotoside (3), trans-melilotoside (4) and dihydromelilotoside (5), two phenylethanoid glycosides verbascoside (6) and galactosylmartynoside (7) and a flavone-C-glycoside, isoorientin (8) were isolated. Many compounds showed moderate to good antiparasitic activity, with isoorientin (8) displaying the most significant antimalarial potential (an IC50 value of 9.7 μg/mL).

Discussion and conclusion: This is the first report on the antiprotozoal evaluation of A. laxmannii extracts and isolates. Furthermore, isoorientin and dihydromelilotoside are being reported for the first time from the genus Ajuga.

Document Type: Article
Keywords: Antiprotozoal activity, flavonoid, iridoid glycosides, Isoorientin, Lamiaceae, plasmodium,
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry
Refereed: Yes
Open Access Journal?: Yes
DOI etc.: 10.3109/13880209.2015.1129542
ISSN: 1388-0209
Date Deposited: 30 Sep 2016 06:49
Last Modified: 01 Feb 2019 15:04
URI: http://oceanrep.geomar.de/id/eprint/34070

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