Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass.

Jueterbock, A., Franssen, S. U., Bergmann, Nina, Gu, J., Coyer, J. A., Reusch, Thorsten B.H. , Bornberg-Bauer, E. and Olsen, J. L. (2016) Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass. Molecular Ecology, 25 (21). pp. 5396-5411. DOI 10.1111/mec.13829.

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Abstract

Populations distributed across a broad thermal cline are instrumental in addressing adaptation to increasing temperatures under global warming. Using a space-for-time substitution design, we tested for parallel adaptation to warm temperatures along two independent thermal clines in Zostera marina, the most widely distributed seagrass in the temperate Northern Hemisphere. A North–South pair of populations was sampled along the European and North American coasts and exposed to a simulated heatwave in a common-garden mesocosm. Transcriptomic responses under control, heat stress and recovery were recorded in 99 RNAseq libraries with ~13 000 uniquely annotated, expressed genes. We corrected for phylogenetic differentiation among populations to discriminate neutral from adaptive differentiation. The two southern populations recovered faster from heat stress and showed parallel transcriptomic differentiation, as compared with northern populations. Among 2389 differentially expressed genes, 21 exceeded neutral expectations and were likely involved in parallel adaptation to warm temperatures. However, the strongest differentiation following phylogenetic correction was between the three Atlantic populations and the Mediterranean population with 128 of 4711 differentially expressed genes exceeding neutral expectations. Although adaptation to warm temperatures is expected to reduce sensitivity to heatwaves, the continued resistance of seagrass to further anthropogenic stresses may be impaired by heat-induced downregulation of genes related to photosynthesis, pathogen defence and stress tolerance.

Document Type: Article
Additional Information: Data accessibility • Supplementary tables (Tables S1–S11) and Appendix S1 are archived at Dryad: http://dx. doi.org/10.5061/dryad.vf5fk. • Supplementary figures (Figs S1–S3) are uploaded as Supporting Information in a single PDF file. • DNA raw reads, the assembled genome sequence and annotation are accessible from NCBI under BioProject number PRJNA41721 with Accession no. LFYR00000000. http://www.ncbi.nlm.nih.gov/bioproject/?LFYR00000000. Further information on the Zostera marina project is available via the Online Resource for Community Annotation Eukaryotes (ORCA) at http://bioinformatics.psb.ugent.be/orcae/ • RNAseq libraries will be made accessible on NCBI under BioProject number PRJNA302837. Integrated School of Ocean Sciences (ISOS), Kiel University
Keywords: common-garden experiment; differential expression; global warming; heatwave; RNAseq; transcriptomics
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1111/mec.13829
ISSN: 0962-1083
Related URLs:
Projects: Zostera marina, AQUASHIFT, BioProject
Date Deposited: 31 Oct 2016 09:25
Last Modified: 01 Feb 2019 15:02
URI: http://oceanrep.geomar.de/id/eprint/34098

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