Acute and chronic nitrite toxicity in juvenile pike-perch (Sander lucioperca) and its compensation by chloride.

Wuertz, S., Schulze, S. G. E., Eberhardt, U., Schulz, Carsten and Schroeder, J. P. (2013) Acute and chronic nitrite toxicity in juvenile pike-perch (Sander lucioperca) and its compensation by chloride. Comparative Biochemistry and Physiology C-Toxicology & Pharmacology, 157 (4). pp. 352-360. DOI 10.1016/j.cbpc.2013.01.002.

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Supplementary data:

Abstract

Pike-perch Sander lucioperca is currently considered as one of the most promising candidates for production in freshwater recirculation aquaculture systems (RAS). Here, due to the lack of studies on nitrite (NO2-) toxicity in pike-perch, a flow-through exposure at 0, 0.44, 0.88, 1.75, 3.5, 7, 14 and 28 mg/L NO2- was carried out to determine the acute and chronic toxicity over a period of 32 days. In juvenile pike-perch, 120 h LC50 was 6.1 mg/L NO2--N and at >= 14 mg/L NO2--N all fish had died within 24 h. Chronic exposure revealed a significant build up of NO2- in the plasma as well as in the muscles at >= 0.44 mg/L NO2--N peaking in fish exposed to the highest concentration of 3.5 mg/L NO2--N after 32 days. Still, due to high individual variation methemoglobin (MetHb) was only significantly increased (p<0.01) at 3.5 mg/L NO2--N. No adverse effects on red blood cells (RBC) and hematocrit were observed in any of the treatments. In a second experiment, compensation of NO2- toxicity at increasing chloride concentrations (40 (freshwater), 65, 90, 140, 240, 440 mg/L Cl-) was observed at a constant exposure of 10 mg/L NO2--N for 42 days. At >= 240 mg/L Cl- NO2- build-up in blood plasma and muscle was completely inhibited. At lower Cl- concentrations (<= 140 mg/L), NO2- was significantly increased in plasma, but only insignificantly elevated in muscle due to high individual variation. MetHb was increased significantly difference only at 40 mg/L Cl- (freshwater control) compared to the control. Again, high individual variations were observed. As a conclusion, S. lucioperca is moderately sensitive towards NO2- and acceptable levels in RAS should hence not exceed 1.75 mg/L NO2--N to avoid MetHb formation. However, based on the 120 h LC50 and a factor of 0.01 according to Sprague (1971), a NO2- concentration of <= 0.061 mg/L NO2--N is considered as "safe." Thereby, no NO2- should accumulate in the plasma or muscle tissue during chronic exposure. For 10 mg/L NO2--N, >= 240 mg/L chloride compensates for NO2- uptake in plasma and muscle. (C) 2013 Elsevier Inc. All rights reserved.

Document Type: Article
Additional Information: Times Cited: 8 Wuertz, S. Schulze, S. G. E. Eberhardt, U. Schulz, C. Schroeder, J. P.
Keywords: Pike-perch, RAS, Nitrite, Toxicity, Histology, Methemoglobin, Methemoglobinemia, Animal welfare, Nitrification
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence > FO-R04
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1016/j.cbpc.2013.01.002
ISSN: 1532-0456
Projects: Future Ocean
Date Deposited: 30 Jan 2017 10:19
Last Modified: 31 Jul 2019 14:40
URI: http://oceanrep.geomar.de/id/eprint/35705

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