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Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.
Baenas, N., Piegholdt, S., Schloesser, A., Moreno, D. A., Garcia-Viguera, C., Rimbach, Gerald and Wagner, A. E. (2016) Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster. International Journal of Molecular Sciences, 17 (2). DOI 10.3390/ijms17020251.
Full text not available from this repository.Abstract
We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPAR-coactivator 1 , as well as the inhibition of -amylase and -glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus.
Document Type: | Article |
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Additional Information: | Times Cited: 0 Baenas, Nieves Piegholdt, Stefanie Schloesser, Anke Moreno, Diego A. Garcia-Viguera, Cristina Rimbach, Gerald Wagner, Anika E. |
Keywords: | Ethiopian rift basalts, Mixed source, Petrogenesis, Veined lithosphere |
Research affiliation: | Kiel University OceanRep > The Future Ocean - Cluster of Excellence |
Refereed: | Yes |
Open Access Journal?: | No |
Publisher: | Elsevier |
Projects: | Future Ocean |
Date Deposited: | 14 Mar 2017 06:26 |
Last Modified: | 23 Sep 2019 21:05 |
URI: | https://oceanrep.geomar.de/id/eprint/36005 |
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