Vasopressin lowers renal epoxyeicosatrienoic acid levels by activating soluble epoxide hydrolase.

Boldt, C., Roschel, T., Himmerkus, N., Plain, A., Bleich, Markus, Labes, R., Blum, M., Krause, H., Magheli, A., Giesecke, T., Mutig, K., Rothe, M., Weldon, S. M., Dragun, D., Schunck, W. H., Bachmann, S. and Paliege, A. (2016) Vasopressin lowers renal epoxyeicosatrienoic acid levels by activating soluble epoxide hydrolase. American Journal of Physiology-Renal Physiology, 311 (6). F1198-F1210. DOI 10.1152/ajprenal.00062.2016.

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Supplementary data:


Activation of the thick ascending limb (TAL) Na+-K+-2Cl(-) cotransporter (NKCC2) by the antidiuretic hormone arginine vasopressin (AVP) is an essential mechanism of renal urine concentration and contributes to extracellular fluid and electrolyte homeostasis. AVP effects in the kidney are modulated by locally and/or by systemically produced epoxyeicosatrienoic acid derivates (EET). The relation between AVP and EET metabolism has not been determined. Here, we show that chronic treatment of AVP-deficient Brattleboro rats with the AVP V2 receptor analog desmopressin (dDAVP; 5 ng/h, 3 days) significantly lowered renal EET levels (-56 +/- 3% for 5,6-EET, -50 +/- 3.4% for 11,12-EET, and -60 +/- 3.7% for 14,15-EET). The abundance of the principal EET-degrading enzyme soluble epoxide hydrolase (sEH) was increased at the mRNA (+160 +/- 37%) and protein levels (+120 +/- 26%). Immunohistochemistry revealed dDAVP-mediated induction of sEH in connecting tubules and cortical and medullary collecting ducts, suggesting a role of these segments in the regulation of local interstitial EET signals. Incubation of murine kidney cell suspensions with 1 mu M 14,15-EET for 30 min reduced phosphorylation of NKCC2 at the AVP-sensitive threonine residues T96 and T101 (-66 +/- 5%; P < 0.05), while 14,15-DHET had no effect. Concomitantly, isolated perfused cortical thick ascending limb pretreated with 14,15-EET showed a 30% lower transport current under high and a 70% lower transport current under low symmetric chloride concentrations. In summary, we have shown that activation of AVP signaling stimulates renal sEH biosynthesis and enzyme activity. The resulting reduction of EET tissue levels may be instrumental for increased NKCC2 transport activity during AVP-induced antidiuresis.

Document Type: Article
Additional Information: Times Cited: 0 Boldt, Christin Roeschel, Tom Himmerkus, Nina Plain, Allein Bleich, Markus Labes, Robert Blum, Maximilian Krause, Hans Magheli, Ahmed Giesecke, Torsten Mutig, Kerim Rothe, Michael Weldon, Steven M. Dragun, Duska Schunck, Wolf-Hagen Bachmann, Sebastian Paliege, Alexander
Keywords: urine concentration mechanism thick ascending limb NKCC2
Research affiliation: Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
Open Access Journal?: No
DOI etc.: 10.1152/ajprenal.00062.2016
ISSN: 1931-857X
Projects: Future Ocean
Date Deposited: 19 Mar 2017 07:48
Last Modified: 23 Sep 2019 20:42

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