Wallner, S., Schroder, C., Leitao, E., Berulava, T., Haak, C., Beisser, D., Rahmann, S., Richter, A. S., Manke, T., Bonisch, U., Arrigoni, L., Frohler, S., Klironomos, F., Chen, W., Rajewsky, N., Muller, F., Ebert, P., Lengauer, T., Barann, M., Rosenstiel, Philip, Gasparoni, G., Nordstrom, K., Walter, J., Brors, B., Zipprich, G., Felder, B., Klein-Hitpass, L., Attenberger, C., Schmitz, G. and Horsthemke, B. (2016) Epigenetic dynamics of monocyte-to-macrophage differentiation. Epigenetics & Chromatin, 9 . DOI 10.1186/s13072-016-0079-z.

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Abstract

Background: Monocyte-to-macrophage differentiation involves major biochemical and structural changes. In order to elucidate the role of gene regulatory changes during this process, we used high-throughput sequencing to analyze the complete transcriptome and epigenome of human monocytes that were differentiated in vitro by addition of colony-stimulating factor 1 in serum-free medium. Results: Numerous mRNAs and miRNAs were significantly up-or down-regulated. More than 100 discrete DNA regions, most often far away from transcription start sites, were rapidly demethylated by the ten eleven translocation enzymes, became nucleosome-free and gained histone marks indicative of active enhancers. These regions were unique for macrophages and associated with genes involved in the regulation of the actin cytoskeleton, phagocytosis and innate immune response. Conclusions: In summary, we have discovered a phagocytic gene network that is repressed by DNA methylation in monocytes and rapidly de-repressed after the onset of macrophage differentiation.

Document Type:	Article
Additional Information:	Times Cited: 2 Wallner, Stefan Schroeder, Christopher Leitao, Elsa Berulava, Tea Haak, Claudia Beisser, Daniela Rahmann, Sven Richter, Andreas S. Manke, Thomas Boenisch, Ulrike Arrigoni, Laura Froehler, Sebastian Klironomos, Filippos Chen, Wei Rajewsky, Nikolaus Mueller, Fabian Ebert, Peter Lengauer, Thomas Barann, Matthias Rosenstiel, Philip Gasparoni, Gilles Nordstroem, Karl Walter, Joern Brors, Benedikt Zipprich, Gideon Felder, Baerbel Klein-Hitpass, Ludger Attenberger, Corinna Schmitz, Gerd Horsthemke, Bernhard
Keywords:	Monocyte, Macrophage, Epigenetics, Methylation, Enhancer, Next-generation sequencing, Ten eleven translocation methylcytosine Dioxygenase, TET, DEEP, IHEC
Research affiliation:	Kiel University Kiel University > Kiel Marine Science OceanRep > The Future Ocean - Cluster of Excellence
Refereed:	Yes
Open Access Journal?:	No
Publisher:	Springer
Projects:	Future Ocean
Date Deposited:	27 Feb 2017 09:30
Last Modified:	23 Sep 2019 23:11
URI:	https://oceanrep.geomar.de/id/eprint/36364

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